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PMID: 1682800 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Sequence and expression of GLN3, a positive nitrogen regulatory gene of Saccharomyces cerevisiae encoding a protein with a putative zinc finger DNA-binding domain.

Molecular and cellular biology ·Vol. 11 ·No. 12 ·1991-12-00 ·Pages 6216-28

Minehart PL, Magasanik B

Abstract

The GLN3 gene of Saccharomyces cerevisiae is required for the activation of transcription of a number of genes in response to the replacement of glutamine by glutamate as source of nitrogen. We cloned the GLN3 gene and constructed null alleles by gene disruption. GLN3 is not essential for growth, but increased copies of GLN3 lead to a drastic decrease in growth rate. The complete nucleotide sequence of the GLN3 gene was determined, revealing one open reading frame encoding a polypeptide of 730 amino acids, with a molecular weight of approximately 80,000. The GLN3 protein contains a single putative Cys2/Cys2 zinc finger which has homology to the Neurospora crassa NIT2 protein, the Aspergillus nidulans AREA protein, and the erythroid-specific transcription factor GATA-1. Immunoprecipitation experiments indicated that the GLN3 protein binds the nitrogen upstream activation sequence of GLN1, the gene encoding glutamine synthetase. Neither control of transcription nor control of initiation of translation of GLN3 is important for regulation in response to glutamine availability.

MeSH Terms
Alleles Amino Acid Sequence Antibodies, Fungal Base Sequence Blotting, Northern Blotting, Western Cloning, Molecular DNA, Fungal/metabolism DNA-Binding Proteins/genetics,immunology,metabolism Fungal Proteins/genetics,immunology,metabolism Gene Expression Regulation, Fungal Glutamate-Ammonia Ligase/genetics Molecular Sequence Data Nitrogen/metabolism Phenotype Plasmids RNA, Fungal/metabolism RNA, Messenger/metabolism Repressor Proteins Restriction Mapping Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transcription Factors Zinc Fingers
Chemicals
Antibodies, Fungal DNA, Fungal DNA-Binding Proteins Fungal Proteins GLN3 protein, S cerevisiae RNA, Fungal RNA, Messenger Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors Glutamate-Ammonia Ligase Nitrogen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Minehart P L
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Magasanik B
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49 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.12.6216-6228.1991
Published
1991-12-00
Pages
6216-28
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361808
Subset
IM
Grants
NIGMS NIH HHS · GM-07446 · United States
Databases
GENBANK
M35267, M64559, M64560, M64561, M64562, M64563, M64564, M64565, M64566, S66430, X59842
External Links
PubMed source DOI: 10.1128/mcb.11.12.6216-6228.1991 PMC Full Text View journal details
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