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PMID: 1652057 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Physiological and genetic analysis of the carbon regulation of the NAD-dependent glutamate dehydrogenase of Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 11 ·No. 9 ·1991-09-00 ·Pages 4455-65

Coschigano PW, Miller SM, Magasanik B

Abstract

We found that cells of Saccharomyces cerevisiae have an elevated level of the NAD-dependent glutamate dehydrogenase (NAD-GDH; encoded by the GDH2 gene) when grown with a nonfermentable carbon source or with limiting amounts of glucose, even in the presence of the repressing nitrogen source glutamine. This regulation was found to be transcriptional, and an upstream activation site (GDH2 UASc) sufficient for activation of transcription during respiratory growth conditions was identified. This UAS was found to be separable from a neighboring element which is necessary for the nitrogen source regulation of the gene, and strains deficient for the GLN3 gene product, required for expression of NAD-GDH during growth with the activating nitrogen source glutamate, were unaffected for the expression of NAD-GDH during growth with activating carbon sources. Two classes of mutations which prevented the normal activation of NAD-GDH in response to growth with nonfermentable carbon sources, but which did not affect the nitrogen-regulated expression of NAD-GDH, were found and characterized. Carbon regulation of GDH2 was found to be normal in hxk2, hap3, and hap4 strains and to be only slightly altered in a ssn6 strain; thus, in comparison with the regulation of previously identified glucose-repressed genes, a new pathway appears to be involved in the regulation of GDH2.

Related Genes
CYC1 GDH2 SUC2
MeSH Terms
Blotting, Northern Carbon/metabolism Cloning, Molecular Cytochrome c Group/genetics Cytochromes c DNA-Binding Proteins Fungal Proteins/metabolism Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Genes, Fungal Glucose/metabolism Glutamate Dehydrogenase/genetics,metabolism Glycoside Hydrolases/genetics Mutation NAD/metabolism Nuclear Proteins Plasmids Repressor Proteins Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins beta-Fructofuranosidase
Chemicals
CYC1 protein, S cerevisiae CYC8 protein, S cerevisiae Cytochrome c Group DNA-Binding Proteins Fungal Proteins Nuclear Proteins Repressor Proteins Saccharomyces cerevisiae Proteins NAD Carbon Cytochromes c Glutamate Dehydrogenase Glycoside Hydrolases beta-Fructofuranosidase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Coschigano P W
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Miller S M
Magasanik B
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.9.4455-4465.1991
Published
1991-09-00
Pages
4455-65
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361309
Subset
IM
Grants
NIGMS NIH HHS · GM-07446 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.9.4455-4465.1991 PMC Full Text View journal details
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