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PMID: 8331070 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis.

Journal of bacteriology ·Vol. 175 ·No. 14 ·1993-07-00 ·Pages 4383-90

Godon JJ, Delorme C, Bardowski J, Chopin MC, Ehrlich SD, Renault P

Abstract

The Lactococcus lactis subsp. lactis strains isolated from dairy products are auxotrophs for branched-chain amino acids (leucine, isoleucine, and valine), while most strains isolated from nondairy media are prototrophs. We have cloned and sequenced the leu genes from one auxotroph, IL1403. The sequence is 99% homologous to that of the prototroph NCDO2118, which was determined previously. Two nonsense mutations and two small deletions were found in the auxotroph sequence, which might explain the branched-chain amino acid auxotrophy. Nevertheless, the leu genes from the auxotroph appear to be transcribed and regulated similarly to those from the prototroph.

Related Genes
leu leuA leuB leuC leuD
MeSH Terms
Amino Acid Sequence Amino Acids, Branched-Chain/biosynthesis Base Sequence Cloning, Molecular DNA, Bacterial/genetics,isolation & purification Escherichia coli/genetics Gene Expression Regulation, Bacterial Genes, Bacterial Lactococcus lactis/genetics,metabolism Molecular Sequence Data Plasmids Transcription, Genetic Transformation, Bacterial
Chemicals
Amino Acids, Branched-Chain DNA, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Godon J J
Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France.
Delorme C
Bardowski J
Chopin M C
Ehrlich S D
Renault P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.14.4383-4390.1993
Published
1993-07-00
Pages
4383-90
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204878
Subset
IM
Databases
GENBANK
M90761
External Links
PubMed source DOI: 10.1128/jb.175.14.4383-4390.1993 PMC Full Text View journal details
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