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PMID: 8335005 Published · ppublish English Journal Article

Small nucleolar RNAs encoded by introns of the human cell cycle regulatory gene RCC1.

The EMBO journal ·Vol. 12 ·No. 7 ·1993-07-00 ·Pages 2913-20

Kiss T, Filipowicz W

Abstract

Eukaryotic cells contain a large number of U small nuclear RNAs (U-snRNAs) involved in various RNA processing reactions in the nucleoplasm and nucleous. Most of the U-snRNAs have 5'-terminal caps added to the end of the primary transcript. Here we describe two variants of a snRNA, called U17, identified in human HeLa cells. U17 RNA may be involved in ribosome biogenesis since it is found in the nucleolus and sediments with 40S structures possibly representing nascent ribosomal subunits. U17 RNAs contain no cap but have a monophosphate at the 5'-terminus indicating that they are processed from longer precursors. The U17 RNAs are encoded within introns 1 and 2 of the single copy gene RCC1 which codes for an important cell cycle regulatory protein. In HeLa cell S-100 extract, U17 RNA is faithfully excised from a longer RNA transcript derived from the intron yielding 5'-monophosphorylated RNA. These data suggest that U17 RNAs are not independently transcribed but are processed out of the RCC1 pre-mRNA or out of the spliced introns.

Related Genes
RCC1
MeSH Terms
Base Sequence Biological Evolution Cell Cycle/genetics Cell Cycle Proteins Cell Nucleolus/metabolism Conserved Sequence DNA-Binding Proteins/genetics Genes, Regulator Guanine Nucleotide Exchange Factors HeLa Cells Humans Introns Molecular Sequence Data Nuclear Proteins/genetics RNA Precursors/metabolism RNA, Small Nuclear/genetics
Chemicals
Cell Cycle Proteins DNA-Binding Proteins Guanine Nucleotide Exchange Factors Nuclear Proteins RCC1 protein, human RNA Precursors RNA, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kiss T
Friedrich Miescher-Institut, Basel, Switzerland.
Filipowicz W
References (43)
43 references, click to expand
  1. Rat nucleolar 7-2 RNA is homologous to mouse mitochondrial RNase mitochondrial RNA-processing RNA.
    J Biol Chem. 1989 Sep 5;264(25):14835-9 PMID: 2475491
  2. A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.
    Nucleic Acids Res. 1988 Aug 25;16(16):8186 PMID: 3047679
  3. Ribocharin: a nuclear Mr 40,000 protein specific to precursor particles of the large ribosomal subunit.
    Cell. 1985 Jun;41(2):615-27 PMID: 3986912
  4. Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae.
    Mol Cell Biol. 1990 Mar;10(3):1145-52 PMID: 2406561
  5. Loss of RCC1, a nuclear DNA-binding protein, uncouples the completion of DNA replication from the activation of cdc2 protein kinase and mitosis.
    EMBO J. 1991 Jun;10(6):1555-64 PMID: 1851087
  6. The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing.
    Cell. 1990 Mar 23;60(6):897-908 PMID: 2156625
  7. Depletion of U3 small nucleolar RNA inhibits cleavage in the 5' external transcribed spacer of yeast pre-ribosomal RNA and impairs formation of 18S ribosomal RNA.
    EMBO J. 1991 Dec;10(13):4231-9 PMID: 1756730
  8. Structure of spliceosomal snRNPs and their role in pre-mRNA splicing.
    Biochim Biophys Acta. 1990 Nov 30;1087(3):265-92 PMID: 2147394
  9. Cell cycle-dependent regulation of histone precursor mRNA processing by modulation of U7 snRNA accessibility.
    Nature. 1990 Aug 16;346(6285):665-8 PMID: 1696685
  10. Nuclear PRP20 protein is required for mRNA export.
    EMBO J. 1993 Jan;12(1):233-41 PMID: 7679070
  11. The RNA processing enzyme RNase MRP is identical to the Th RNP and related to RNase P.
    Science. 1989 Sep 22;245(4924):1377-80 PMID: 2476849
  12. Analysis of pre-mRNA processing in transfected plant protoplasts.
    Methods Enzymol. 1990;181:148-61 PMID: 1696344
  13. Plant small nuclear RNAs. Nucleolar U3 snRNA is present in plants: partial characterization.
    Eur J Biochem. 1985 Oct 15;152(2):259-66 PMID: 4054108
  14. Modification of the 5' end of mRNA. Association of RNA triphosphatase with the RNA guanylyltransferase-RNA (guanine-7-)methyltransferase complex from vaccinia virus.
    J Biol Chem. 1980 Feb 10;255(3):903-8 PMID: 6243301
  15. Isolation and characterization of the active cDNA of the human cell cycle gene (RCC1) involved in the regulation of onset of chromosome condensation.
    Genes Dev. 1987 Aug;1(6):585-93 PMID: 3678831
  16. Evidence against a mitochondrial location of the 7-2/MRP RNA in mammalian cells.
    Cell. 1992 Jul 10;70(1):11-6 PMID: 1377982
  17. Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1.
    Nature. 1991 Nov 7;354(6348):80-2 PMID: 1944575
  18. Mouse U14 snRNA is a processed intron of the cognate hsc70 heat shock pre-messenger RNA.
    Cell. 1992 Dec 24;71(7):1215-21 PMID: 1282084
  19. RCC1 in the cell cycle: the regulator of chromosome condensation takes on new roles.
    Trends Biochem Sci. 1993 Mar;18(3):96-101 PMID: 8480369
  20. Characterization of an SNR gene locus in Saccharomyces cerevisiae that specifies both dispensible and essential small nuclear RNAs.
    Mol Cell Biol. 1988 Aug;8(8):3282-90 PMID: 2850487
  21. Origin of splice junction phosphate in tRNAs processed by HeLa cell extract.
    Cell. 1983 Feb;32(2):547-57 PMID: 6186399
  22. Complete nucleotide sequence of the human RCC1 gene involved in coupling between DNA replication and mitosis.
    Genomics. 1991 Oct;11(2):459-61 PMID: 1769659
  23. U3, U8 and U13 comprise a new class of mammalian snRNPs localized in the cell nucleolus.
    EMBO J. 1989 Oct;8(10):3113-9 PMID: 2531075
  24. Specific labeling of 3' termini of RNA with T4 RNA ligase.
    Methods Enzymol. 1980;65(1):65-74 PMID: 6154874
  25. Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5' ends of mRNAs and for constructing cDNA libraries by in vitro amplification.
    Nucleic Acids Res. 1991 Oct 11;19(19):5227-32 PMID: 1923806
  26. Gamma-monomethyl phosphate: a cap structure in spliceosomal U6 small nuclear RNA.
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8280-3 PMID: 2813391
  27. Mitochondrial RNA synthesis during mitosis.
    Science. 1970 Apr 3;168(3927):135-8 PMID: 5417056
  28. Formation of a 2'-phosphomonoester, 3',5'-phosphodiester linkage by a novel RNA ligase in wheat germ.
    Nature. 1981 Sep 10-16;293(5828):112-6 PMID: 7266664
  29. Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase.
    Cell. 1991 Jul 26;66(2):347-60 PMID: 1855255
  30. The maternally expressed Drosophila gene encoding the chromatin-binding protein BJ1 is a homolog of the vertebrate gene Regulator of Chromatin Condensation, RCC1.
    EMBO J. 1991 May;10(5):1225-36 PMID: 2022188
  31. Mouse U14 snRNA is encoded in an intron of the mouse cognate hsc70 heat shock gene.
    Nucleic Acids Res. 1990 Nov 25;18(22):6565-71 PMID: 2251119
  32. An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
    EMBO J. 1991 Sep;10(9):2645-51 PMID: 1714385
  33. Checkpoint controls that couple mitosis to completion of DNA replication.
    Trends Cell Biol. 1992 Sep;2(9):277-81 PMID: 14731521
  34. A yeast mutant, PRP20, altered in mRNA metabolism and maintenance of the nuclear structure, is defective in a gene homologous to the human gene RCC1 which is involved in the control of chromosome condensation.
    Mol Gen Genet. 1990 Oct;224(1):72-80 PMID: 2277633
  35. A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA.
    EMBO J. 1987 Dec 20;6(13):4169-75 PMID: 3327689
  36. 7-2/MRP RNAs in plant and mammalian cells: association with higher order structures in the nucleolus.
    EMBO J. 1992 Oct;11(10):3737-46 PMID: 1382978
  37. Three small nucleolar RNAs of unique nucleotide sequences.
    Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):635-8 PMID: 8421699
  38. Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitro.
    Cell. 1991 May 3;65(3):517-26 PMID: 1826860
  39. Defects in mRNA 3'-end formation, transcription initiation, and mRNA transport associated with the yeast mutation prp20: possible coupling of mRNA processing and chromatin structure.
    Genes Dev. 1992 Oct;6(10):1914-26 PMID: 1398069
  40. Regulation of synthesis and processing of nucleolar components in metaphase-arrested cells.
    J Mol Biol. 1971 Jul 14;59(1):27-42 PMID: 5283755
  41. In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing.
    EMBO J. 1990 Jul;9(7):2299-308 PMID: 2357971
  42. Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.
    Cell. 1981 Nov;27(1 Pt 2):165-74 PMID: 7326749
  43. Yeast snR30 is a small nucleolar RNA required for 18S rRNA synthesis.
    Mol Cell Biol. 1993 Apr;13(4):2469-77 PMID: 8455623
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-07-00
Pages
2913-20
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413544
Subset
IM
Databases
GENBANK
D00591
External Links
PubMed source PMC Full Text View journal details
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