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PMID: 8355713 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Saccharomyces cerevisiae BUF protein binds to sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation.

Molecular and cellular biology ·Vol. 13 ·No. 9 ·1993-09-00 ·Pages 5749-61

Luche RM, Smart WC, Marion T, Tillman M, Sumrada RA, Cooper TG

Abstract

The heteromeric BUF protein was originally shown to bind to URS1 elements which are situated upstream of many genes in Saccharomyces cerevisiae and mediate negative control of their transcription. Among the genes regulated through the URS1 site and the proteins interacting with it are those participating in carbon, nitrogen, and inositol metabolism; electron transport; meiosis; sporulation; and mating-type switching. We show here that pure BUF protein, in addition to binding to the negatively acting URS1 site, also binds to CAR1 sequences supporting transcriptional activation (upstream activation sequences). To determine the BUF protein structure, we cloned and sequenced the BUF1 and BUF2 genes and found them to be identical to the RF-A (RP-A) gene whose products participate in yeast DNA replication as single-stranded DNA binding proteins. These data argue that BUF protein-binding sites serve multiple roles in transcription and replication.

Related Genes
BUF1 BUF2 RF-A RP-A
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Replication DNA, Fungal/biosynthesis DNA-Binding Proteins/genetics,isolation & purification,metabolism Gene Expression Regulation, Fungal Genes, Fungal Genes, Lethal Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Replication Protein A Repressor Proteins/genetics Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Transcription Factors/genetics,metabolism Transcription, Genetic Zinc Fingers
Chemicals
DNA, Fungal DNA-Binding Proteins Oligodeoxyribonucleotides RFA1 protein, S cerevisiae RFA2 protein, S cerevisiae Replication Protein A Repressor Proteins Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Luche R M
Department of Microbiology and Immunology, University of Tennessee, Memphis 38163.
Smart W C
Marion T
Tillman M
Sumrada R A
Cooper T G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.9.5749-5761.1993
Published
1993-09-00
Pages
5749-61
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360314
Subset
IM
Grants
NIGMS NIH HHS · R01 GM035642 · United States
NIGMS NIH HHS · GM 35642 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.9.5749-5761.1993 PMC Full Text View journal details
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