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PMID: 8367294 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transcriptional repressor ZF5 identifies a new conserved domain in zinc finger proteins.

Nucleic acids research ·Vol. 21 ·No. 16 ·1993-08-11 ·Pages 3767-75

Numoto M, Niwa O, Kaplan J, Wong KK, Merrell K, Kamiya K, Yanagihara K, Calame K

Abstract

We have cloned a cDNA encoding a new murine C2H2 zinc finger protein, ZF5. The 51.3 kD protein contains five GL1-Kruppel type zinc fingers at the C-terminus. At its N-terminus, ZF5 has a 41 amino acid region which was found to be homologous to the N-termini of several other zinc finger proteins. This region defines a new motif within zinc finger proteins which we have named the Zinc finger N-terminal (ZiN) domain. ZF5 binds to two sites in the c-myc promoter and to the -50 bp site of the herpes simplex thymidine kinase promoter. ZF5 is a transcriptional repressor and its repression domain is located N-terminal to the zinc finger domains. A single 4 kb ZF5 mRNA is expressed widely.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Binding, Competitive Cell Line Cloning, Molecular Conserved Sequence DNA/isolation & purification DNA-Binding Proteins Mice Molecular Sequence Data Promoter Regions, Genetic Proto-Oncogene Proteins c-myc/genetics RNA, Messenger/biosynthesis Repressor Proteins/chemistry,genetics Sequence Homology, Amino Acid Thymidine Kinase/genetics Transcription, Genetic Transfection Tumor Cells, Cultured Zinc Fingers/genetics
Chemicals
DNA-Binding Proteins Proto-Oncogene Proteins c-myc RNA, Messenger Repressor Proteins Zfp161 protein, mouse DNA Thymidine Kinase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Numoto M
Research Institute for Nuclear Medicine and Biology, Hiroshima University, Japan.
Niwa O
Kaplan J
Wong K K
Merrell K
Kamiya K
Yanagihara K
Calame K
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.16.3767
Published
1993-08-11
Pages
3767-75
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309887
Subset
IM
Grants
NCI NIH HHS · CA 38571 · United States
NIGMS NIH HHS · GM07367 · United States
Databases
GENBANK
L15325
External Links
PubMed source DOI: 10.1093/nar/21.16.3767 PMC Full Text View journal details
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