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PMID: 8387995 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Binding of an Escherichia coli double-stranded DNA virus PRD1 to a receptor coded by an IncP-type plasmid.

Journal of bacteriology ·Vol. 175 ·No. 10 ·1993-05-00 ·Pages 3089-95

Kotilainen MM, Grahn AM, Bamford JK, Bamford DH

Abstract

IncP plasmid RP1 Tra regions are needed to assemble the receptor for lipid-containing double-stranded DNA bacteriophage PRD1 on the cell surface. Using radioactively labeled phage and electron microscopic techniques, we showed that the surfaces of Salmonella typhimurium(RP1) and Escherichia coli(RP1) cells contained approximately 50 and 20 PRD1 binding sites, respectively. Expression of the receptor was growth phase dependent and was highest at late logarithmic or early stationary phase. The PRD1-resistant RP1 transposon mutants isolated were all Tra-, and the transposons were located in both the Tra1 and Tra2 regions.

MeSH Terms
Coliphages/drug effects,growth & development DNA Mutational Analysis DNA Transposable Elements Escherichia coli/genetics,metabolism,ultrastructure Lipopolysaccharides/pharmacology Microscopy, Electron Mutagenesis, Insertional Negative Staining Plasmids/genetics Receptors, Virus/genetics,metabolism Salmonella typhimurium/drug effects,metabolism,ultrastructure
Chemicals
DNA Transposable Elements Lipopolysaccharides Receptors, Virus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kotilainen M M
Department of Genetics, University of Helsinki, Finland.
Grahn A M
Bamford J K
Bamford D H
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30 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.10.3089-3095.1993
Published
1993-05-00
Pages
3089-95
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204630
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.175.10.3089-3095.1993 PMC Full Text View journal details
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