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PMID: 8389925 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

cis-acting elements in the lytic origin of DNA replication of Epstein-Barr virus.

Journal of virology ·Vol. 67 ·No. 7 ·1993-07-00 ·Pages 4237-45

Schepers A, Pich D, Mankertz J, Hammerschmidt W

Abstract

oriLyt, the cis-acting element of Epstein-Barr virus, mediates viral DNA replication in the lytic phase of the virus's life cycle. Oligonucleotide-directed in vitro mutagenesis of oriLyt plasmids allowed the identification of two noncontiguous components within the complex structure of oriLyt. Both components were indispensable for DNA replication of this origin. The upstream component colocalized with the promoter of the viral BHLF1-encoding gene, and mutants affecting DNA replication affected RNA transcription, too. The second component crucial for oriLyt function was determined to be 40 bp long and positioned approximately 530 bp downstream. It was dispensable for transcriptional transactivation but it was absolutely required for replication. Thus, the overall design of oriLyt has striking similarity to multipartite regulatory elements of transcription, consisting of proximal promoters and distal enhancers, but special elements are exclusively dedicated to DNA replication.

MeSH Terms
Base Sequence Cell Line DNA Replication DNA, Viral/genetics Gene Expression Regulation, Viral Herpesvirus 4, Human/genetics Humans Molecular Sequence Data Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Restriction Mapping Transcription, Genetic Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schepers A
Institut für Klinische Molekularbiologie und Tumorgenetik, GSF-Forschungszentrum für Umwelt und Gesundheit, GmbH, München, Germany.
Pich D
Mankertz J
Hammerschmidt W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.7.4237-4245.1993
Published
1993-07-00
Pages
4237-45
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237793
Subset
IM
Grants
NIAID NIH HHS · AI-29988 · United States
NCI NIH HHS · CA-07175 · United States
NCI NIH HHS · CA-22443 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.7.4237-4245.1993 PMC Full Text View journal details
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