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PMID: 8392630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Deletion of DNA encoding the first five transmembrane domains of Epstein-Barr virus latent membrane proteins 2A and 2B.

Journal of virology ·Vol. 67 ·No. 8 ·1993-08-00 ·Pages 5068-74

Longnecker R, Miller CL, Tomkinson B, Miao XQ, Kieff E

Abstract

A recombinant Epstein-Barr virus (EBV) was constructed, with a positive-selection marker inserted at the site of a deletion of a DNA segment which encodes the first five transmembrane domains of LMP2A and LMP2B. Despite the mutation, the mutant recombinant EBV was able to initiate and maintain primary B-lymphocyte growth transformation in vitro. Cells transformed with the mutant recombinant were not different from wild-type virus transformants in initial or long-term outgrowth, sensitivity to limiting cell dilution, or serum requirement. Expression of EBNA1, EBNA2, EBNA3A, EBNA3C, and LMP1 and permissivity for lytic EBV infection were also unaffected by the LMP2 deletion mutation. These results complete the molecular genetic studies proving LMP2 is dispensable for primary B-lymphocyte growth transformation, latent infection, and lytic virus replication in vitro.

Related Genes
EBNA1 LMP1 LMP2A LMP2B
MeSH Terms
Amino Acid Sequence Antigens, Viral/biosynthesis,genetics B-Lymphocytes/microbiology Base Sequence Blotting, Southern Cell Transformation, Viral DNA, Viral/genetics,metabolism Gene Expression Genes, Viral Herpesvirus 4, Human/genetics,metabolism Humans Lymphocyte Activation Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction Recombination, Genetic Restriction Mapping Sequence Deletion Transcription, Genetic Viral Matrix Proteins/biosynthesis,genetics Virus Replication
Chemicals
Antigens, Viral DNA, Viral EBV-associated membrane antigen, Epstein-Barr virus Oligodeoxyribonucleotides Viral Matrix Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Longnecker R
Department of Medicine, Harvard Medical School, Boston, Massachusetts 02115.
Miller C L
Tomkinson B
Miao X Q
Kieff E
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35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.8.5068-5074.1993
Published
1993-08-00
Pages
5068-74
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237899
Subset
IM
Grants
NCI NIH HHS · CA 47006 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.8.5068-5074.1993 PMC Full Text View journal details
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