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PMID: 8406814 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of novel type C staphylococcal enterotoxins: biological and evolutionary implications.

Infection and immunity ·Vol. 61 ·No. 10 ·1993-10-00 ·Pages 4254-62

Marr JC, Lyon JD, Roberson JR, Lupher M, Davis WC, Bohach GA

Abstract

The type C staphylococcal enterotoxins (SEC) are a group of highly conserved proteins with significant immunological cross-reactivity. Although three antigenically distinct SEC subtypes (SEC1, SEC2, and SEC3) have been reported in the literature, we observed that the isoelectric points of SEC from several Staphylococcus aureus isolates are different from those of any of these three subtypes. This observation led us to propose that additional SEC molecular variants exist. For assessment of this possibility, the sec genes from representative human, animal, and food isolates were cloned and sequenced. The toxins encoded by the 18 isolates used in this study included five unique SEC proteins in addition to SEC1, SEC2, and SEC3. Six of the SEC proteins (including SEC1, SEC2, and SEC3) were produced by human and food isolates. Analysis of seven bovine and ovine isolates showed that isolates from each animal species produced a unique host-specific SEC. All of the SEC caused lymphocyte proliferation, although some of the toxins differed in their ability to stimulate cells from several animal species. An explanation for these results, which is supported by our phenotypic analysis of Sec+ staphylococcal isolates, is that toxin heterogeneity is due to selection for modified SEC sequences that facilitate the survival of S. aureus isolates in their respective hosts.

Related Genes
sec
MeSH Terms
Amino Acid Sequence Bacterial Toxins/chemistry Base Sequence Cloning, Molecular Enterotoxins/chemistry Genes, Bacterial Lymphocyte Activation/drug effects Molecular Sequence Data Oligodeoxyribonucleotides/chemistry Restriction Mapping Sequence Alignment Serotyping Staphylococcus/chemistry,genetics,pathogenicity
Chemicals
Bacterial Toxins Enterotoxins Oligodeoxyribonucleotides
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Marr J C
Department of Bacteriology and Biochemistry, University of Idaho, Moscow 83843.
Lyon J D
Roberson J R
Lupher M
Davis W C
Bohach G A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.10.4254-4262.1993
Published
1993-10-00
Pages
4254-62
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC281152
Subset
IM
Grants
NIAID NIH HHS · AI28401 · United States
Databases
GENBANK
L13374, L13375, L13376, L13377, L13378, L13379
External Links
PubMed source DOI: 10.1128/iai.61.10.4254-4262.1993 PMC Full Text View journal details
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