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PMID: 8407851 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Absence of a direct role for RNase HI in initiation of DNA replication at the oriC site on the Escherichia coli chromosome.

Journal of bacteriology ·Vol. 175 ·No. 20 ·1993-10-00 ·Pages 6731-4

Hong X, Kogoma T

Abstract

On the basis of the experiments carried out with rnhA224 mutants, we previously concluded that RNase HI is not essential for initiation of Escherichia coli chromosome replication at oriC (T. Kogoma, N.L. Subia, and K. von Meyenburg, Mol. Gen. Genet. 200:103-109, 1985). In light of the recent finding that rnhA224 is a UGA nonsense mutation which can be leaky in certain genetic backgrounds, we reexamined this conclusion with the use of rnhA339 (Null)::cat mutants. The possibility that recB+ is required for initiation at the alternative origins (oriKs) of replication in rnhA mutants was also tested. The results clearly indicated that RNase HI is not essential for oriC initiation and that recB+ is not required for initiation at oriK sites.

Related Genes
oriC
MeSH Terms
Chromosomes, Bacterial/metabolism DNA Replication DNA, Bacterial/biosynthesis Escherichia coli/genetics Regulatory Sequences, Nucleic Acid Ribonuclease H/metabolism
Chemicals
DNA, Bacterial Ribonuclease H ribonuclease HI
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hong X
Department of Cell Biology, University of New Mexico School of Medicine, Albuquerque 87131.
Kogoma T
References (22)
22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.20.6731-6734.1993
Published
1993-10-00
Pages
6731-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206788
Subset
IM
Grants
NIGMS NIH HHS · GM22092 · United States
External Links
PubMed source DOI: 10.1128/jb.175.20.6731-6734.1993 PMC Full Text View journal details
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