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PMID: 8417335 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

An essential yeast gene with homology to the exonuclease-encoding XRN1/KEM1 gene also encodes a protein with exoribonuclease activity.

Molecular and cellular biology ·Vol. 13 ·No. 1 ·1993-01-00 ·Pages 341-50

Kenna M, Stevens A, McCammon M, Douglas MG

Abstract

An essential gene, designated HKE1/RAT1, has been isolated from the yeast Saccharomyces cerevisiae and characterized. The gene encodes a protein of 116 kDa (p116) and has significant homology to another yeast gene (XRN1/KEM1) encoding a related protein (p175) with 5'-->3' exonuclease activity as well as activities involving chromosomal DNA pairing and mechanics. Preliminary analysis of an hke1ts mutant reveals a precipitous decline in the translation of mRNA at the nonpermissive temperature. Sporulation of heterozygous HKE1/hke1::URA3 diploids reveals that this gene, unlike the highly related XRN1/KEM1 gene, is essential for cell viability. Overexpression of the homologous gene product, p175, failed to rescue cells lacking a functional p116. In vitro studies demonstrate that p116 is a protein with 5'-->3' exoribonuclease activity, a major activity of the related p175. An immunoreactive RNase activity of 116 kDa is abolished with antiserum against p116. Both the level of this protein and the RNase activity correlate with HKE1 gene dosage. The RNase activity purifies coincidentally with a previously described 116-kDa RNase having 5'-->3' exoribonuclease activity.

Related Genes
HKE1 KEM1 RAT1 XRN1
MeSH Terms
Cloning, Molecular Exoribonucleases/genetics Fungal Proteins/genetics Genes, Fungal Genetic Complementation Test Immunologic Techniques Mutagenesis, Insertional Protein Biosynthesis Saccharomyces cerevisiae/enzymology,genetics
Chemicals
Fungal Proteins Exoribonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kenna M
Department of Biochemistry and Biophysics, University of North Carolina Medical School, Chapel Hill 27599-7260.
Stevens A
McCammon M
Douglas M G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.1.341-350.1993
Published
1993-01-00
Pages
341-50
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358913
Subset
IM
Grants
NIGMS NIH HHS · GM41758 · United States
External Links
PubMed source DOI: 10.1128/mcb.13.1.341-350.1993 PMC Full Text View journal details
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