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PMID: 8421704 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Developmental regulation of a murine T-cell-specific tyrosine kinase gene, Tsk.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 2 ·1993-01-15 ·Pages 669-73

Heyeck SD, Berg LJ

Abstract

Protein-tyrosine kinases have been implicated in signal transduction in T lymphocytes after stimulation of many cell-surface molecules, including the T-cell antigen receptor, CD4, CD8, CD2, CD5, and CD28. Yet the identities of many of these tyrosine kinases remain unknown. We have isolated a murine tyrosine kinase gene, called Tsk for T-cell-specific kinase, that appears to be exclusively expressed in T lymphocytes. The Tsk cDNA clone encodes a polypeptide of 70 kDa, which is similar in sequence to both the src and abl families of tyrosine kinases. Sequence comparisons also indicate that Tsk contains one src-homology region 2 domain and one src-homology 3 domain but lacks the negative regulatory tyrosine (src Tyr-527) common to src-family kinases. In addition, Tsk expression is developmentally regulated. Steady-state Tsk mRNA levels are 5- to 10-fold higher in thymocytes than in peripheral T cells and increase in the thymus during mouse development from neonate to adult. Furthermore, Tsk is expressed in day 14 fetal thymus, suggesting a role for Tsk in early T-lymphocyte differentiation.

Related Genes
Tsk
MeSH Terms
Aging Amino Acid Sequence Animals Animals, Newborn Base Sequence Cell Differentiation/physiology Cloning, Molecular Gene Expression Regulation Genes, abl/genetics Genes, src/genetics Mice Molecular Sequence Data Molecular Weight Polymerase Chain Reaction Protein-Tyrosine Kinases/genetics Signal Transduction/physiology T-Lymphocytes/enzymology Thymus Gland Tissue Distribution
Chemicals
Protein-Tyrosine Kinases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Heyeck S D
Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.
Berg L J
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.2.669
Published
1993-01-15
Pages
669-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC45725
Subset
IM
Databases
GENBANK
L05631
External Links
PubMed source DOI: 10.1073/pnas.90.2.669 PMC Full Text View journal details
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