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PMID: 8455607 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Relatedness of an RNA-binding motif in human immunodeficiency virus type 1 TAR RNA-binding protein TRBP to human P1/dsI kinase and Drosophila staufen.

Molecular and cellular biology ·Vol. 13 ·No. 4 ·1993-04-00 ·Pages 2193-202

Gatignol A, Buckler C, Jeang KT

Abstract

TRBP is a human cellular protein that binds the human immunodeficiency virus type 1 TAR RNA. Here, we show that the intact presence of amino acids 247 to 267 in TRBP correlates with its ability to bind RNA. This region contains a lysine- and arginine-rich motif, KKLAKRNAAAKMLLRVHTVPLDAR. A 24-amino-acid synthetic peptide (TR1) of this sequence bound TAR RNA with affinities similar to that of the entire TRBP, thus suggesting that this short motif contains a sufficient RNA-binding activity. Using RNA probe-shift analysis, we determined that TR1 does not bind all double-stranded RNAs but prefers TAR and other double-stranded RNAs with G+C-rich characteristics. Immunoprecipitation of TRBP from human immunodeficiency virus type 1-infected T lymphocytes recovered TAR RNA. This is consistent with a TRBP-TAR ribonucleoprotein during viral infection. Computer alignment revealed that TR1 is highly homologous to the RNA-binding domain of human P1/dsI protein kinase and two regions within Drosophila Staufen. We suggest that these proteins are related by virtue of sharing a common RNA-binding moiety.

Related Genes
Staufen TRBP
MeSH Terms
Amino Acid Sequence Base Sequence Consensus Sequence DNA Mutational Analysis Genes HIV-1/genetics HeLa Cells Humans Hydrogen Bonding Molecular Sequence Data Peptides/chemistry,metabolism RNA, Viral/metabolism,ultrastructure RNA-Binding Proteins/genetics Sequence Alignment Sequence Deletion T-Lymphocytes/metabolism,microbiology
Chemicals
Peptides RNA, Viral RNA-Binding Proteins trans-activation responsive RNA-binding protein
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gatignol A
Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
Buckler C
Jeang K T
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.4.2193-2202.1993
Published
1993-04-00
Pages
2193-202
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359540
Subset
IM
Databases
GENBANK
M35663, M60725, M60801, M69111, M85294, M96370, X02946
External Links
PubMed source DOI: 10.1128/mcb.13.4.2193-2202.1993 PMC Full Text View journal details
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