Home LiteratureArticle Details
PMID: 8474451 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mouse beta-globin DNA-binding protein B1 is identical to a proto-oncogene, the transcription factor Spi-1/PU.1, and is restricted in expression to hematopoietic cells and the testis.

Molecular and cellular biology ·Vol. 13 ·No. 5 ·1993-05-00 ·Pages 2929-41

Galson DL, Hensold JO, Bishop TR, Schalling M, D'Andrea AD, Jones C, Auron PE, Housman DE

Abstract

The hematopoietic-specific DNA-binding protein B1 binds to the DNA consensus sequence AAAGRGGAARYG located twice in intervening sequence 2 of both of the mouse beta-globin genes (D. L. Galson and D.E. Housman, Mol. Cell. Biol. 8:381-392, 1988). B1 was cloned by expression of a murine erythroleukemia (MEL) cell cDNA library in transfected COS cells and screening by electrophoretic mobility shift analysis. B1 is identical to the proto-oncogene Spi-1/PU.1 (Spi-1), an ets family member. Protein-DNA contacts are shown to resemble those of the helix-turn-helix homeodomain proteins. By Northern (RNA) analysis, we found that Spi-1 mRNA is present at low levels during murine CFU-E maturation and is at least 20-fold higher in uninduced MEL, a transformed proerythroblast-like cell line which contains an activating/transforming insertion of spleen focus-forming virus at the Spi-1 locus. Dimethyl sulfoxide-induced MEL cell differentiation decreases Spi-1 mRNA to approximately 20% of the uninduced level before commitment occurs. In addition to erythroid cells, Spi-1 mRNA is present in B cells, myelomonocytes, and mast cells but not in T cells and nonhematopoietic cell types. In situ hybridization demonstrated Spi-1 mRNA expression in bone marrow, spleen, interstitial nonhepatocytes of the liver, and interstitial nontubular cells of the testis. The Spi-1 locus was mapped on human chromosome 11 to the same interval as ACP2 (lysosomal acid phosphatase), between the anonymous DNA markers D11S33 and D11S14. This region has not yet been found to be associated with a human malignancy.

Related Genes
Spi-1 ets
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Chromosome Mapping Chromosomes, Human, Pair 11 DNA/genetics,metabolism DNA-Binding Proteins/genetics,metabolism Erythrocytes/physiology Gene Expression Globins/genetics Hematopoietic Stem Cells/physiology Humans In Situ Hybridization Leukemia, Erythroblastic, Acute/genetics Male Mice Mice, Inbred BALB C Molecular Sequence Data Multigene Family Oncogenes Organ Specificity Proto-Oncogene Mas Proto-Oncogenes RNA, Messenger/genetics,metabolism Retroviridae Proteins, Oncogenic/genetics Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Spleen/physiology Testis/physiology Transcription Factors/genetics,metabolism Transfection Tumor Cells, Cultured
Chemicals
DNA-Binding Proteins MAS1 protein, human Proto-Oncogene Mas RNA, Messenger Retroviridae Proteins, Oncogenic Transcription Factors oncogene proteins v-ets v-Spi-1 protein, Friend spleen focus-forming virus Globins DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Galson D L
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139.
Hensold J O
Bishop T R
Schalling M
D'Andrea A D
Jones C
Auron P E
Housman D E
References (81)
81 references, click to expand
  1. Isolation and induction of erythroleukemic cell lines with properties of erythroid progenitor burst-forming cell (BFU-E) and erythroid precursor cell (CFU-E).
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3721-5 PMID: 6574510
  2. DNase I hypersensitive sites of globin genes of uninduced Friend erythroleukemia cells and changes during induction with dimethyl sulfoxide.
    J Biol Chem. 1983 Sep 10;258(17):10622-8 PMID: 6224791
  3. Genomic sequencing.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5 PMID: 6326095
  4. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  5. Rapid transfer of DNA from agarose gels to nylon membranes.
    Nucleic Acids Res. 1985 Oct 25;13(20):7207-21 PMID: 4059056
  6. Nucleosomes are phased along the mouse beta-major globin gene in erythroid and nonerythroid cells.
    Cell. 1986 Mar 14;44(5):697-704 PMID: 3081263
  7. Regulated expression of human A gamma-, beta-, and hybrid gamma beta-globin genes in transgenic mice: manipulation of the developmental expression patterns.
    Cell. 1986 Jul 4;46(1):89-94 PMID: 3719696
  8. The beta-subunit of follicle-stimulating hormone is deleted in patients with aniridia and Wilms' tumour, allowing a further definition of the WAGR locus.
    Nature. 1986 Jun 26-Jul 2;321(6073):882-7 PMID: 3014343
  9. Regulation of human interleukin-2 gene: functional DNA sequences in the 5' flanking region for the gene expression in activated T lymphocytes.
    Cell. 1986 Aug 1;46(3):401-5 PMID: 3015413
  10. Localization of the human oncogene SPI1 on chromosome 11, region p11.22.
    Hum Genet. 1990 May;84(6):542-6 PMID: 2338340
  11. The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors.
    Oncogene. 1990 May;5(5):663-8 PMID: 1693183
  12. DNA binding properties of the purified Antennapedia homeodomain.
    Proc Natl Acad Sci U S A. 1990 Jun;87(11):4093-7 PMID: 1971945
  13. The PU.1 transcription factor is the product of the putative oncogene Spi-1.
    Cell. 1990 Jun 29;61(7):1165-6 PMID: 2364426
  14. DNA recognition by proteins with the helix-turn-helix motif.
    Annu Rev Biochem. 1990;59:933-69 PMID: 2197994
  15. Improved detection of helix-turn-helix DNA-binding motifs in protein sequences.
    Nucleic Acids Res. 1990 Sep 11;18(17):5019-26 PMID: 2402433
  16. Crystal structure of an engrailed homeodomain-DNA complex at 2.8 A resolution: a framework for understanding homeodomain-DNA interactions.
    Cell. 1990 Nov 2;63(3):579-90 PMID: 1977522
  17. Analysis of expression of cholecystokinin in dopamine cells in the ventral mesencephalon of several species and in humans with schizophrenia.
    Proc Natl Acad Sci U S A. 1990 Nov;87(21):8427-31 PMID: 1978324
  18. Role of tryptophan repeats and flanking amino acids in Myb-DNA interactions.
    Proc Natl Acad Sci U S A. 1990 Nov;87(21):8452-6 PMID: 2236054
  19. The ETS-domain: a new DNA-binding motif that recognizes a purine-rich core DNA sequence.
    Genes Dev. 1990 Sep;4(9):1451-3 PMID: 2253872
  20. The Sfpi-1 proviral integration site of Friend erythroleukemia encodes the ets-related transcription factor Pu.1.
    J Virol. 1991 Jan;65(1):464-7 PMID: 1985210
  21. Calcium ionophore, A23187, induces commitment to differentiation but inhibits the subsequent expression of erythroid genes in murine erythroleukemia cells.
    Blood. 1991 Mar 15;77(6):1362-70 PMID: 2001459
  22. Erythroleukemia induction by Friend murine leukemia virus: insertional activation of a new member of the ets gene family, Fli-1, closely linked to c-ets-1.
    Genes Dev. 1991 Jun;5(6):908-18 PMID: 2044959
  23. Expression of the Wilms' tumor gene WT1 in the murine urogenital system.
    Genes Dev. 1991 Aug;5(8):1345-56 PMID: 1651275
  24. Convergence of Ets- and notch-related structural motifs in a heteromeric DNA binding complex.
    Science. 1991 Aug 16;253(5021):762-8 PMID: 1876833
  25. Identification of Ets- and notch-related subunits in GA binding protein.
    Science. 1991 Aug 16;253(5021):789-92 PMID: 1876836
  26. Specific DNA binding by c-Myb: evidence for a double helix-turn-helix-related motif.
    Science. 1991 Sep 6;253(5024):1140-3 PMID: 1887237
  27. Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3.
    Cell. 1986 Oct 10;47(1):3-10 PMID: 3489530
  28. Expression of chimeric human beta- and delta-globin genes during erythroid differentiation.
    J Biol Chem. 1987 Apr 5;262(10):4819-26 PMID: 3470293
  29. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
    Anal Biochem. 1987 Apr;162(1):156-9 PMID: 2440339
  30. Rapid alkaline blot-transfer of viral dsRNAs.
    Anal Biochem. 1987 May 15;163(1):210-8 PMID: 3039868
  31. Detection of two tissue-specific DNA-binding proteins with affinity for sites in the mouse beta-globin intervening sequence 2.
    Mol Cell Biol. 1988 Jan;8(1):381-92 PMID: 3422099
  32. Spi-1 is a putative oncogene in virally induced murine erythroleukaemias.
    Nature. 1988 Jan 21;331(6153):277-80 PMID: 2827041
  33. Identification, purification, and characterization of a mast cell-associated cytolytic factor related to tumor necrosis factor.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9175-9 PMID: 3321069
  34. A purine-rich DNA sequence motif present in SV40 and lymphotropic papovavirus binds a lymphoid-specific factor and contributes to enhancer activity in lymphoid cells.
    Genes Dev. 1987 Nov;1(9):962-72 PMID: 2828177
  35. Patterns of differential gene expression during maturation of erythroid colony forming units.
    Prog Clin Biol Res. 1987;251:43-54 PMID: 3432273
  36. Expression of the human beta-globin gene after retroviral transfer into murine erythroleukemia cells and human BFU-E cells.
    Mol Cell Biol. 1988 Apr;8(4):1725-35 PMID: 3288863
  37. Nuclease hypersensitive sites in chromatin.
    Annu Rev Biochem. 1988;57:159-97 PMID: 3052270
  38. A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
    Cell. 1989 Mar 10;56(5):777-83 PMID: 2493990
  39. Interaction of murine ets-1 with GGA-binding sites establishes the ETS domain as a new DNA-binding motif.
    Genes Dev. 1992 Jun;6(6):975-90 PMID: 1592264
  40. Role of the PU.1 transcription factor in controlling differentiation of Friend erythroleukemia cells.
    Mol Cell Biol. 1992 Jul;12(7):2967-75 PMID: 1620109
  41. Transcription factors: structural families and principles of DNA recognition.
    Annu Rev Biochem. 1992;61:1053-95 PMID: 1497306
  42. The proto-oncogene PU.1 regulates expression of the myeloid-specific CD11b promoter.
    J Biol Chem. 1993 Mar 5;268(7):5014-20 PMID: 8095266
  43. Genetics of somatic mammalian cells: genetic, immunologic, and biochemical analysis with Chinese hamster cell hybrids containing selected human chromosomes.
    Proc Natl Acad Sci U S A. 1976 Jan;73(1):193-7 PMID: 1061117
  44. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  45. Erythroleukemic differentiation.
    Annu Rev Biochem. 1978;47:419-48 PMID: 354501
  46. Molecular analysis of cloned human 18S ribosomal DNA segments.
    Proc Natl Acad Sci U S A. 1978 Nov;75(11):5367-71 PMID: 364477
  47. Regional mapping of the gene for human lysosomal acid phosphatase (ACP2) using a hybrid clone panel containing segments of human chromosome 11.
    Hum Genet. 1978 Nov 24;45(1):1-10 PMID: 730175
  48. Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):122-6 PMID: 6987648
  49. Number and evolutionary conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned cDNA probes.
    Cell. 1980 May;20(1):95-105 PMID: 6893015
  50. Inducer T lymphocytes synthesize a factor that stimulates proliferation of cloned mast cells.
    Nature. 1981 May 28;291(5813):332-4 PMID: 6972009
  51. Murine erythroleukemia cell differentiation: DNase I hypersensitivity and DNA methylation near the globin genes.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):1180-4 PMID: 6280172
  52. Terminal differentiation of murine erythroleukemia cells: physical stabilization of end-stage cells.
    J Cell Biol. 1982 May;93(2):390-4 PMID: 6954153
  53. Mast cell clones: a model for the analysis of cellular maturation.
    J Cell Biol. 1982 Nov;95(2 Pt 1):435-44 PMID: 6216259
  54. Globin RNA transcription: a possible termination site and demonstration of transcriptional control correlated with altered chromatin structure.
    Cell. 1982 Jul;29(3):887-93 PMID: 7151174
  55. Commitment of murine erythroleukemia (MEL) cells to terminal differentiation is associated with coordinated expression of globin and ribosomal genes.
    Prog Clin Biol Res. 1982;102 pt A:69-79 PMID: 6300921
  56. Expression cloning of the murine erythropoietin receptor.
    Cell. 1989 Apr 21;57(2):277-85 PMID: 2539263
  57. Cloning of cDNA for the major DNA-binding protein of the erythroid lineage through expression in mammalian cells.
    Nature. 1989 Jun 8;339(6224):446-51 PMID: 2725678
  58. Interleukin 3-dependent and -independent mast cells stimulated with IgE and antigen express multiple cytokines.
    J Exp Med. 1989 Jul 1;170(1):245-57 PMID: 2473161
  59. The structure and function of the homeodomain.
    Biochim Biophys Acta. 1989 Jul 28;989(1):25-48 PMID: 2568852
  60. Eukaryotic transcriptional regulatory proteins.
    Annu Rev Biochem. 1989;58:799-839 PMID: 2673023
  61. Report of the committee on the genetic constitution of chromosome 11.
    Cytogenet Cell Genet. 1989;51(1-4):226-58 PMID: 2676371
  62. DNA-binding domain ancestry.
    Nature. 1989 Nov 9;342(6246):134 PMID: 2572967
  63. Molecular biology of Friend viral erythroleukemia.
    Curr Top Microbiol Immunol. 1989;148:1-42 PMID: 2684547
  64. Definition of an Ets1 protein domain required for nuclear localization in cells and DNA-binding activity in vitro.
    Mol Cell Biol. 1989 Dec;9(12):5718-21 PMID: 2555704
  65. The putative oncogene Spi-1: murine chromosomal localization and transcriptional activation in murine acute erythroleukemias.
    Oncogene. 1989 Dec;4(12):1449-56 PMID: 2594367
  66. A fine-structure deletion map of human chromosome 11p: analysis of J1 series hybrids.
    Somat Cell Mol Genet. 1989 Nov;15(6):477-501 PMID: 2595451
  67. Spi-1 oncogene activation in Rauscher and Friend murine virus-induced acute erythroleukemias.
    Leukemia. 1990 Jan;4(1):20-3 PMID: 2153262
  68. Complete physical map of the WAGR region of 11p13 localizes a candidate Wilms' tumor gene.
    Cell. 1990 Feb 9;60(3):495-508 PMID: 2154334
  69. A panel of irradiation-reduced hybrids selectively retaining human chromosome 11p13: their structure and use to purify the WAGR gene complex.
    Genomics. 1990 Jan;6(1):48-64 PMID: 2154397
  70. Identification and mapping of a common proviral integration site Fli-1 in erythroleukemia cells induced by Friend murine leukemia virus.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1332-6 PMID: 2304901
  71. Conserved residues make similar contacts in two repressor-operator complexes.
    Science. 1990 Mar 9;247(4947):1210-3 PMID: 2315694
  72. The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene.
    Cell. 1990 Apr 6;61(1):113-24 PMID: 2180582
  73. Cell-type-specific control elements of the lymphotropic papovavirus enhancer.
    J Virol. 1990 Apr;64(4):1657-66 PMID: 2319649
  74. Friend virus-induced erythroleukemia and the multistage nature of cancer.
    Cell. 1991 Sep 6;66(5):831-4 PMID: 1889087
  75. Crystal structure of a MAT alpha 2 homeodomain-operator complex suggests a general model for homeodomain-DNA interactions.
    Cell. 1991 Nov 1;67(3):517-28 PMID: 1682054
  76. Ets-related protein Elk-1 is homologous to the c-fos regulatory factor p62TCF.
    Nature. 1991 Dec 19-26;354(6354):531-4 PMID: 1722028
  77. PU.1 recruits a second nuclear factor to a site important for immunoglobulin kappa 3' enhancer activity.
    Mol Cell Biol. 1992 Jan;12(1):368-78 PMID: 1729611
  78. Characterization of SAP-1, a protein recruited by serum response factor to the c-fos serum response element.
    Cell. 1992 Feb 7;68(3):597-612 PMID: 1339307
  79. cis-acting sequences required for inducible interleukin-2 enhancer function bind a novel Ets-related protein, Elf-1.
    Mol Cell Biol. 1992 Mar;12(3):1043-53 PMID: 1545787
  80. Evolutionarily conserved Ets family members display distinct DNA binding specificities.
    J Exp Med. 1992 May 1;175(5):1391-9 PMID: 1569404
  81. Onset of erythropoietin response in murine erythroid colony-forming units: assignment to early S-phase in a specific cell generation.
    Blood. 1992 May 15;79(10):2749-58 PMID: 1316785
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.5.2929-2941.1993
Published
1993-05-00
Pages
2929-41
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359686
Subset
IM
Grants
NCI NIH HHS · CA-18724 · United States
NIDDK NIH HHS · DK-39888 · United States
NCI NIH HHS · P30-CA-43703 · United States
Databases
GENBANK
L03215
External Links
PubMed source DOI: 10.1128/mcb.13.5.2929-2941.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2