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PMID: 8478098 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Molecular characterization of a leukotoxin gene from a Pasteurella haemolytica-like organism, encoding a new member of the RTX toxin family.

Infection and immunity ·Vol. 61 ·No. 5 ·1993-05-00 ·Pages 2089-95

Chang YF, Ma DP, Shi J, Chengappa MM

Abstract

A Pasteurella haemolytica-like organism, a new species of bacterium isolated from piglets with diarrhea, secretes a leukotoxin into the culture media. Western blot (immunoblot) analysis indicated that this leukotoxin cross-reacted with antileukotoxin antibody derived from cattle immunized with P. haemolytica. Five overlapping recombinant bacteriophages carrying the gene for this 105-kDa polypeptide were identified with a DNA probe containing sequences from the P. haemolytica lktCA genes from a P. haemolytica-like organism strain 5943 genomic library. Sequence analysis of a region of the cloned DNA revealed two open reading frames encoding proteins with predicted masses of 19.4 and 101.6 kDa. These genes, which we designate pllktC (P. haemolytica-like organism leukotoxin C gene) and pllktA (A gene), respectively, are similar in sequence to the RTX (repeat of toxin) toxin family. The structure of the 101.6-kDa protein derived from the DNA sequence shows three transmembrane domains in the N-terminal part of the protein, 13 glycine-rich repeat domains in the second half of the protein, and a hydrophobic C-terminal part. pllktC and pllktA are strongly homologous to P. haemolytica lktC and lktA genes. However, this leukotoxin kills both BL-3 and pig leukocytes and is not hemolytic.

Related Genes
pllktA pllktC
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Western Cloning, Molecular Cytotoxins/genetics DNA, Bacterial/immunology Diarrhea/microbiology,veterinary Exotoxins/genetics,immunology,toxicity Genes, Bacterial Molecular Sequence Data Pasteurella/genetics Recombinant Proteins/toxicity Sequence Alignment Swine Swine Diseases/microbiology
Chemicals
Cytotoxins DNA, Bacterial Exotoxins Recombinant Proteins leukotoxin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Chang Y F
Diagnostic Laboratory, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.
Ma D P
Shi J
Chengappa M M
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.61.5.2089-2095.1993
Published
1993-05-00
Pages
2089-95
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC280807
Subset
IM
Databases
GENBANK
L12148
External Links
PubMed source DOI: 10.1128/iai.61.5.2089-2095.1993 PMC Full Text View journal details
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