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PMID: 8550448 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Amplification of a novel gene, sanA, abolishes a vancomycin-sensitive defect in Escherichia coli.

Journal of bacteriology ·Vol. 178 ·No. 1 ·1996-01-00 ·Pages 94-102

Rida S, Caillet J, Alix JH

Abstract

We have isolated an Escherichia coli gene which, when overexpressed, is able to complement the permeability defects of a vancomycin-susceptible mutant. This gene, designated sanA, is located at min 47 of the E. coli chromosome and codes for a 20-kDa protein with a highly hydrophobic amino-terminal segment. A strain carrying a null mutation of the sanA gene, transferred to the E. coli chromosome by homologous recombination, is perfectly viable, but after two generations at high temperature (43 degrees C), the barrier function of its envelope towards vancomycin is defective.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/pharmacology Bacterial Outer Membrane Proteins/analysis Base Sequence Cell Membrane Permeability Cloning, Molecular Detergents/pharmacology Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics,growth & development Escherichia coli Proteins Genes, Bacterial/genetics Hot Temperature Membrane Proteins/biosynthesis,genetics,metabolism Molecular Sequence Data Mutation Restriction Mapping Sequence Analysis, DNA Sodium Dodecyl Sulfate/pharmacology Vancomycin/pharmacology
Chemicals
Anti-Bacterial Agents Bacterial Outer Membrane Proteins Detergents Escherichia coli Proteins Membrane Proteins SanA protein, E coli Sodium Dodecyl Sulfate Vancomycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rida S
Institut de Biologie Physico-Chimique, Paris, France.
Caillet J
Alix J H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.178.1.94-102.1996
Published
1996-01-00
Pages
94-102
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177625
Subset
IM
Databases
GENBANK
Z47804
External Links
PubMed source DOI: 10.1128/jb.178.1.94-102.1996 PMC Full Text View journal details
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