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PMID: 8157594 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Assembly of LamB and OmpF in deep rough lipopolysaccharide mutants of Escherichia coli K-12.

Journal of bacteriology ·Vol. 176 ·No. 8 ·1994-04-00 ·Pages 2259-64

Laird MW, Kloser AW, Misra R

Abstract

Assembly of the OmpF and LamB proteins was kinetically retarded in deep rough lipopolysaccharide mutants of Escherichia coli K-12. OmpF assembly was affected at the step of conversion of metastable trimers to stable trimers, whereas LamB assembly was influenced both at the monomer-to-metastable trimer and metastable-to-stable trimer steps. These assembly defects were reversed in the presence of the sfaA1 and sfaB3 suppressor alleles, which were isolated by using ompF assembly mutants.

Related Genes
rfa sfa
MeSH Terms
Bacterial Outer Membrane Proteins/biosynthesis,genetics Escherichia coli/genetics,metabolism Kinetics Lipopolysaccharides/metabolism Mutation/genetics,physiology Porins Receptors, Virus/biosynthesis,genetics
Chemicals
Bacterial Outer Membrane Proteins Lipopolysaccharides Porins Receptors, Virus maltoporins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laird M W
Department of Microbiology, Arizona State University, Tempe 85287-2701.
Kloser A W
Misra R
References (28)
28 references, click to expand
  1. Maltose transport in Escherichia coli K-12: involvement of the bacteriophage lambda receptor.
    J Bacteriol. 1975 Oct;124(1):112-8 PMID: 1100596
  2. A genetic approach for analyzing the pathway of LamB assembly into the outer membrane of Escherichia coli.
    J Biol Chem. 1991 Jul 25;266(21):13592-7 PMID: 1856196
  3. Outer membrane of Salmonella. Isolation of protein complex that produces transmembrane channels.
    J Biol Chem. 1976 Apr 10;251(7):2176-8 PMID: 773934
  4. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.
    J Mol Biol. 1976 Jul 5;104(3):541-55 PMID: 781293
  5. Assembly pathway of newly synthesized LamB protein an outer membrane protein of Escherichia coli K-12.
    J Mol Biol. 1984 Jun 5;175(4):511-28 PMID: 6204059
  6. Molecular basis of bacterial outer membrane permeability.
    Microbiol Rev. 1985 Mar;49(1):1-32 PMID: 2580220
  7. Bacteriophage K20 requires both the OmpF porin and lipopolysaccharide for receptor function.
    J Bacteriol. 1987 Oct;169(10):4830-3 PMID: 2820945
  8. Genetics and biochemistry of the assembly of proteins into the outer membrane of E. coli.
    Prog Biophys Mol Biol. 1987;49(2-3):89-115 PMID: 3327100
  9. Targeting of porin to the outer membrane of Escherichia coli. Rate of trimer assembly and identification of a dimer intermediate.
    J Biol Chem. 1988 Jun 5;263(16):7753-9 PMID: 2836396
  10. Structure and function of porins from gram-negative bacteria.
    Annu Rev Microbiol. 1988;42:359-93 PMID: 2849372
  11. Role of the rfaG and rfaP genes in determining the lipopolysaccharide core structure and cell surface properties of Escherichia coli K-12.
    J Bacteriol. 1992 Apr;174(8):2525-38 PMID: 1348243
  12. Dynamics of the exposure of epitopes on OmpF, an outer membrane protein of Escherichia coli.
    Eur J Biochem. 1992 May 15;206(1):109-14 PMID: 1375155
  13. OmpF assembly mutants of Escherichia coli K-12: isolation, characterization, and suppressor analysis.
    J Bacteriol. 1993 Aug;175(16):5049-56 PMID: 8349546
  14. Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12.
    Cell. 1981 Dec;27(3 Pt 2):507-14 PMID: 6086106
  15. Genetics of lipopolysaccharide biosynthesis in enteric bacteria.
    Microbiol Rev. 1993 Sep;57(3):655-82 PMID: 7504166
  16. Isolation of the bacteriophage lambda receptor from Escherichia coli.
    J Bacteriol. 1973 Dec;116(3):1436-46 PMID: 4201774
  17. Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations.
    J Bacteriol. 1974 Feb;117(2):406-16 PMID: 4590466
  18. Alterations in the outer membrane of the cell envelope of heptose-deficient mutants of Escherichia coli.
    J Bacteriol. 1974 Feb;117(2):527-43 PMID: 4590475
  19. Asymmetrical distribution and artifactual reorientation of lipopolysaccharide in the outer membrane bilayer of Salmonella typhimurium.
    Eur J Biochem. 1975 Feb 21;51(2):343-52 PMID: 807474
  20. The assembly of the major outer membrane protein OmpF of Escherichia coli depends on lipid synthesis.
    EMBO J. 1988 Nov;7(11):3595-9 PMID: 3061802
  21. rfaP mutants of Salmonella typhimurium.
    Eur J Biochem. 1989 Nov 20;185(3):541-6 PMID: 2686988
  22. In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli.
    Proc Natl Acad Sci U S A. 1990 Jan;87(2):743-7 PMID: 1689050
  23. In vitro folding and oligomerization of a membrane protein. Transition of bacterial porin from random coil to native conformation.
    J Biol Chem. 1990 Jun 25;265(18):10217-20 PMID: 1693917
  24. Associations of Escherichia coli K-12 OmpF trimers with rough and smooth lipopolysaccharides.
    J Bacteriol. 1990 Sep;172(9):5307-11 PMID: 2168378
  25. Role of lipopolysaccharide in assembly of Escherichia coli outer membrane proteins OmpA, OmpC, and OmpF.
    J Bacteriol. 1990 Oct;172(10):6048-53 PMID: 2170338
  26. Lipopolysaccharide structure required for in vitro trimerization of Escherichia coli OmpF porin.
    J Bacteriol. 1991 Jan;173(2):926-8 PMID: 1702785
  27. Trimerization of an in vitro synthesized OmpF porin of Escherichia coli outer membrane.
    J Biol Chem. 1991 Jun 15;266(17):11295-300 PMID: 2040634
  28. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
    FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.8.2259-2264.1994
Published
1994-04-00
Pages
2259-64
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205347
Subset
IM
Grants
NIGMS NIH HHS · GM48167 · United States
External Links
PubMed source DOI: 10.1128/jb.176.8.2259-2264.1994 PMC Full Text View journal details
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