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PMID: 8557750 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

V-src kinase shifts the cadherin-based cell adhesion from the strong to the weak state and beta catenin is not required for the shift.

The Journal of cell biology ·Vol. 131 ·No. 6 Pt 2 ·1995-12-00 ·Pages 1839-47

Takeda H, Nagafuchi A, Yonemura S, Tsukita S, Behrens J, Birchmeier W, Tsukita S

Abstract

The elevation of tyrosine phosphorylation level is thought to induce the dysfunction of cadherin through the tyrosine phosphorylation of beta catenin. We evaluated this assumption using two cell lines. First, using temperature-sensitive v-src-transfected MDCK cells, we analyzed the modulation of cadherin-based cell adhesion by tyrosine phosphorylation. Cell aggregation and dissociation assays at nonpermissive and permissive temperatures indicated that elevation of the tyrosine phosphorylation does not totally affect the cell adhesion ability of cadherin but shifts it from a strong to a weak state. The tyrosine phosphorylation levels of beta catenin, ZO-1, ERM (ezrin/radixin/moesin), but not alpha catenin, vinculin, and alpha-actinin, were elevated in the weak state. To evaluate the involvement of the tyrosine phosphorylation of beta catenin in this shift of cadherin-based cell adhesion, we introduced v-src kinase into L fibroblasts expressing the cadherin-alpha catenin fusion protein, in which beta catenin is not involved in cell adhesion. The introduction of v-src kinase in these cells shifted their adhesion from a strong to a weak state. These findings indicated that the tyrosine phosphorylation of beta catenin is not required for the strong-to-weak state shift of cadherin-based cell adhesion, but that the tyrosine phosphorylation of other junctional proteins, ERM, ZO-1 or unidentified proteins is involved.

MeSH Terms
Animals Cadherins/metabolism Cell Adhesion/physiology Cell Aggregation/physiology Cell Line/enzymology Cytoskeletal Proteins/metabolism Dogs Kidney Tubules, Distal/cytology Mice Oncogene Protein pp60(v-src)/metabolism Phosphorylation Precipitin Tests Recombinant Fusion Proteins/metabolism Temperature Trans-Activators Tyrosine/metabolism beta Catenin
Chemicals
CTNNB1 protein, mouse Cadherins Cytoskeletal Proteins Recombinant Fusion Proteins Trans-Activators beta Catenin Tyrosine Oncogene Protein pp60(v-src)
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Takeda H
Department of Information Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Nagafuchi A
Yonemura S
Tsukita S
Behrens J
Birchmeier W
Tsukita S
References (46)
46 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Association of p120, a tyrosine kinase substrate, with E-cadherin/catenin complexes.
    J Cell Biol. 1995 Mar;128(5):949-57 PMID: 7876318
  3. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells.
    Cell. 1975 Nov;6(3):289-98 PMID: 802682
  4. A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.
    Cell. 1979 Sep;18(1):193-205 PMID: 574428
  5. Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus.
    Cell. 1981 Apr;24(1):165-74 PMID: 6263485
  6. Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells.
    Cell. 1981 Jun;24(3):741-52 PMID: 6166387
  7. Vinculin phosphorylation by the src kinase. Interaction of vinculin with phospholipid vesicles.
    J Biol Chem. 1983 Dec 10;258(23):14626-31 PMID: 6417142
  8. Role of tyrosine phosphorylation in malignant transformation by viruses and in cellular growth control.
    Prog Nucleic Acid Res Mol Biol. 1983;29:221-32 PMID: 6686688
  9. Overexpressed pp60c-src can induce focus formation without complete transformation of NIH 3T3 cells.
    Mol Cell Biol. 1985 May;5(5):1073-83 PMID: 2582237
  10. Some structural and functional aspects of the cell adhesion molecule uvomorulin.
    Cell Differ. 1984 Dec;15(2-4):269-73 PMID: 6336035
  11. Characterization of antigens recognized by monoclonal and polyclonal antibodies directed against uvomorulin.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8067-71 PMID: 2415979
  12. The protein-tyrosine kinase substrate, p81, is homologous to a chicken microvillar core protein.
    J Cell Biol. 1986 Feb;102(2):660-9 PMID: 2418035
  13. Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.
    J Cell Biol. 1986 Sep;103(3):755-66 PMID: 3528172
  14. Functional correlation between cell adhesive properties and some cell surface proteins.
    J Cell Biol. 1977 Nov;75(2 Pt 1):464-74 PMID: 264120
  15. Microvillus-specific Mr 75,000 plasma membrane protein of human choriocarcinoma cells.
    J Histochem Cytochem. 1987 Aug;35(8):809-16 PMID: 3298422
  16. Nonmitogenic morphoregulatory action of pp60v-src on multicellular epithelial structures.
    Mol Cell Biol. 1987 Apr;7(4):1326-37 PMID: 3110593
  17. Expression and distribution of cell adhesion molecule uvomorulin in mouse preimplantation embryos.
    Dev Biol. 1987 Dec;124(2):451-6 PMID: 3315781
  18. Characterization of ZO-1, a protein component of the tight junction from mouse liver and Madin-Darby canine kidney cells.
    J Cell Biol. 1988 Apr;106(4):1141-9 PMID: 2452168
  19. Cellular transformation, tyrosine kinase oncogenes, and the cellular adhesion plaque.
    Bioessays. 1988 Jan;8(1):25-30 PMID: 3130051
  20. Phosphotyrosine-modified proteins are concentrated at the membranes of epithelial and endothelial cells during tissue development in chick embryos.
    J Cell Biol. 1988 May;106(5):1757-64 PMID: 2453522
  21. Expressed recombinant cadherins mediate cell sorting in model systems.
    Cell. 1988 Sep 23;54(7):993-1001 PMID: 3416359
  22. The cadherins: cell-cell adhesion molecules controlling animal morphogenesis.
    Development. 1988 Apr;102(4):639-55 PMID: 3048970
  23. Rapid phosphorylation and reorganization of ezrin and spectrin accompany morphological changes induced in A-431 cells by epidermal growth factor.
    J Cell Biol. 1989 Mar;108(3):921-30 PMID: 2646308
  24. A new 82-kD barbed end-capping protein (radixin) localized in the cell-to-cell adherens junction: purification and characterization.
    J Cell Biol. 1989 Jun;108(6):2369-82 PMID: 2500445
  25. The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species.
    EMBO J. 1989 Jun;8(6):1711-7 PMID: 2788574
  26. Phosphorylation of the tight-junction protein ZO-1 in two strains of Madin-Darby canine kidney cells which differ in transepithelial resistance.
    Biochem J. 1989 Oct 15;263(2):597-9 PMID: 2597123
  27. Cadherin cell adhesion receptors as a morphogenetic regulator.
    Science. 1991 Mar 22;251(5000):1451-5 PMID: 2006419
  28. Specific proto-oncogenic tyrosine kinases of src family are enriched in cell-to-cell adherens junctions where the level of tyrosine phosphorylation is elevated.
    J Cell Biol. 1991 May;113(4):867-79 PMID: 1709169
  29. The 102 kd cadherin-associated protein: similarity to vinculin and posttranscriptional regulation of expression.
    Cell. 1991 May 31;65(5):849-57 PMID: 1904011
  30. Modulation of intercellular adherens-type junctions and tyrosine phosphorylation of their components in RSV-transformed cultured chick lens cells.
    Cell Regul. 1991 Feb;2(2):105-20 PMID: 1650581
  31. A 220-kD undercoat-constitutive protein: its specific localization at cadherin-based cell-cell adhesion sites.
    J Cell Biol. 1991 Dec;115(5):1449-62 PMID: 1955485
  32. The effect of tyrosine-specific protein phosphorylation on the assembly of adherens-type junctions.
    EMBO J. 1992 May;11(5):1733-42 PMID: 1316270
  33. Cadherin-mediated cell-cell adhesion is perturbed by v-src tyrosine phosphorylation in metastatic fibroblasts.
    J Cell Biol. 1992 Aug;118(3):703-14 PMID: 1639852
  34. Identification of the two major epidermal growth factor-induced tyrosine phosphorylation sites in the microvillar core protein ezrin.
    J Biol Chem. 1992 Sep 25;267(27):19258-65 PMID: 1382070
  35. Molecular linkage between cadherins and actin filaments in cell-cell adherens junctions.
    Curr Opin Cell Biol. 1992 Oct;4(5):834-9 PMID: 1419062
  36. Loss of epithelial differentiation and gain of invasiveness correlates with tyrosine phosphorylation of the E-cadherin/beta-catenin complex in cells transformed with a temperature-sensitive v-SRC gene.
    J Cell Biol. 1993 Feb;120(3):757-66 PMID: 8425900
  37. p60v-src causes tyrosine phosphorylation and inactivation of the N-cadherin-catenin cell adhesion system.
    EMBO J. 1993 Jan;12(1):307-14 PMID: 8381351
  38. The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy.
    J Cell Biol. 1993 May;121(3):491-502 PMID: 8486731
  39. Submembranous junctional plaque proteins include potential tumor suppressor molecules.
    J Cell Biol. 1993 Dec;123(5):1049-53 PMID: 8245116
  40. Relationship of serine/threonine phosphorylation/dephosphorylation signaling to glucocorticoid regulation of tight junction permeability and ZO-1 distribution in nontransformed mammary epithelial cells.
    J Biol Chem. 1994 Jun 10;269(23):16108-15 PMID: 8206910
  41. Dynamics of cadherin/catenin complex formation: novel protein interactions and pathways of complex assembly.
    J Cell Biol. 1994 Jun;125(6):1327-40 PMID: 8207061
  42. Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells.
    J Cell Sci. 1995 Apr;108 ( Pt 4):1735-42 PMID: 7542259
  43. Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members.
    J Cell Biol. 1994 Jun;125(6):1371-84 PMID: 8207064
  44. The roles of catenins in the cadherin-mediated cell adhesion: functional analysis of E-cadherin-alpha catenin fusion molecules.
    J Cell Biol. 1994 Oct;127(1):235-45 PMID: 7929566
  45. Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor.
    J Cell Biol. 1994 Dec;127(5):1375-80 PMID: 7962096
  46. Cell shape and membrane changes in the eight-cell mouse embryo: prerequisites for morphogenesis of the blastocyst.
    Dev Biol. 1975 Nov;47(1):45-58 PMID: 173595
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.131.6.1839
Published
1995-12-00
Pages
1839-47
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120684
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.131.6.1839 PMC Full Text View journal details
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