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PMID: 8668161 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Association of p300 and CBP with simian virus 40 large T antigen.

Molecular and cellular biology ·Vol. 16 ·No. 7 ·1996-07-00 ·Pages 3454-64

Eckner R, Ludlow JW, Lill NL, Oldread E, Arany Z, Modjtahedi N, DeCaprio JA, Livingston DM, Morgan JA

Abstract

p300 and the CREB-binding protein CBP are two large nuclear phosphoproteins that are structurally highly related. Both function, in part, as transcriptional adapters and are targeted by the adenovirus E1A oncoprotein. We show here that p300 and CBP interact with another transforming protein, the simian virus 40 large T antigen (T). This interaction depends on the integrity of a region of T which is critical for its transforming and mitogenic properties and includes its LXCXE Rb-binding motif. T interferes with normal p300 and CBP function on at least two different levels. The presence of T alters the phosphorylation states of both proteins and inhibits their transcriptional activities on certain promoters. Although E1A and T show little sequence similarity, they interact with the same domain of p300 and CBP, suggesting that this region exhibits considerable flexibility in accommodating diverse protein ligands.

MeSH Terms
Amino Acid Sequence Animals Antibodies Antigens, Polyomavirus Transforming/isolation & purification,metabolism Base Sequence Binding Sites Blotting, Western CREB-Binding Protein Cell Division E1A-Associated p300 Protein Electrophoresis, Polyacrylamide Gel Embryo, Mammalian Fibroblasts Glutathione Transferase Mice Molecular Sequence Data Mutagenesis, Site-Directed Nuclear Proteins/biosynthesis,isolation & purification,metabolism Oligodeoxyribonucleotides Phosphoproteins/metabolism Phosphorylation Recombinant Fusion Proteins/biosynthesis,isolation & purification,metabolism Trans-Activators Transcription Factors/biosynthesis,isolation & purification,metabolism Transfection
Chemicals
Antibodies Antigens, Polyomavirus Transforming Nuclear Proteins Oligodeoxyribonucleotides Phosphoproteins Recombinant Fusion Proteins Trans-Activators Transcription Factors CREB-Binding Protein Crebbp protein, mouse E1A-Associated p300 Protein Ep300 protein, mouse Glutathione Transferase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Eckner R
Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.
Ludlow J W
Lill N L
Oldread E
Arany Z
Modjtahedi N
DeCaprio J A
Livingston D M
Morgan J A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.7.3454
Published
1996-07-00
Pages
3454-64
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231340
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.7.3454 PMC Full Text View journal details
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