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PMID: 8675293 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Iron starvation causes release from the group A streptococcus of the ADP-ribosylating protein called plasmin receptor or surface glyceraldehyde-3-phosphate-dehydrogenase.

Infection and immunity ·Vol. 64 ·No. 6 ·1996-06-00 ·Pages 1956-60

Eichenbaum Z, Green BD, Scott JR

Abstract

In many pathogenic bacteria, iron starvation serves as an environmental signal that triggers the expression of virulence factors, many of which are found on the cell surface or secreted into the culture supernatant. Using the chelating agent nitrilotriacetic acid, we have established conditions for iron starvation of the important human pathogen Streptococcus pyogenes (the group A streptococcus) and determined that iron limitation results in the specific appearance of several new proteins in the culture supernatant. One of these supernatant proteins is the ADP-ribosylating protein known as streptococcal plasmin receptor (Plr) or as the streptococcal surface glyceraldehyde-3-phosphate-dehydrogenase because of its other activities. Upon iron starvation, Plr is specifically released into the culture supernatant in a time-dependent manner, and its appearance in the supernatant is not accompanied by induction of plr mRNA synthesis. Release of Plr from the bacteria may be important for the virulence of group A streptococci and the manifestation of diseases.

MeSH Terms
Adenosine Diphosphate Ribose/metabolism Base Sequence Glyceraldehyde-3-Phosphate Dehydrogenases/metabolism Humans Iron/physiology Molecular Sequence Data Molecular Weight Receptors, Peptide/metabolism Streptococcus pyogenes/growth & development,metabolism
Chemicals
Receptors, Peptide plasmin receptor Adenosine Diphosphate Ribose Iron Glyceraldehyde-3-Phosphate Dehydrogenases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Eichenbaum Z
Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30322, USA.
Green B D
Scott J R
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.64.6.1956-1960.1996
Published
1996-06-00
Pages
1956-60
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC174022
Subset
IM
Grants
NIAID NIH HHS · R37 AI 20723 · United States
External Links
PubMed source DOI: 10.1128/iai.64.6.1956-1960.1996 PMC Full Text View journal details
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