Home LiteratureArticle Details
PMID: 8707827 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

gamma-Tubulin-like Tub4p of Saccharomyces cerevisiae is associated with the spindle pole body substructures that organize microtubules and is required for mitotic spindle formation.

The Journal of cell biology ·Vol. 134 ·No. 2 ·1996-07-00 ·Pages 429-41

Spang A, Geissler S, Grein K, Schiebel E

Abstract

Tub4p is a novel tubulin in Saccharomyces cerevisiae that most closely resembles gamma-tubulin. We report in this manuscript that the essential Tub4p is associated with the inner and outer plaques of the yeast microtubule organizing center, the spindle pole body (SPB). These SPB substructures are involved in the attachment of the nuclear and cytoplasmic microtubules, respectively (Byers, B., and L. Goetsch. 1975. J. Bacteriol. 124:511-523). Study of a temperature sensitive tub4-1 allele revealed that TUB4 has essential functions in microtubule organization. Remarkably, SPB duplication and separation are not impaired in tub4-1 cells incubated at the nonpermissive temperature. However, SPBs from such cells contain less or misdirected nuclear microtubules. Further analysis revealed that tub4-1 cells are able to assemble a short bipolar spindle, suggesting that the defect in microtubule organization occurs after spindle formation. A role of Tub4p in microtubule organization is further suggested by an increase in chromosome loss in tub4-1 cells. In addition, cell cycle arrest and survival of tub4-1 cells is dependent on the mitotic checkpoint control gene BUB2 (Hoyt, M.A., L. Totis, B.T. Roberts. 1991. Cell. 66:507-517), one of the cell's monitors of spindle integrity.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Cycle/genetics Cell Cycle Proteins Chromosomes, Fungal DNA, Fungal Fungal Proteins/genetics,metabolism Genetic Complementation Test Microtubules/metabolism Molecular Sequence Data Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Signal Transduction Spindle Apparatus/metabolism Tubulin/genetics,metabolism Xenopus laevis
Chemicals
BUB2 protein, S cerevisiae Cell Cycle Proteins DNA, Fungal Fungal Proteins Saccharomyces cerevisiae Proteins TUB4 protein, S cerevisiae Tubulin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Spang A
Max-Planck-Institut für Biochemie, Genzentrum, Martinsried, Germany.
Geissler S
Grein K
Schiebel E
References (42)
42 references, click to expand
  1. Microbial determinations by flow cytometry.
    J Gen Microbiol. 1979 Aug;113(2):369-75 PMID: 390094
  2. The role of spindle pole bodies and modified microtubule ends in the initiation of microtubule assembly in Saccharomyces cerevisiae.
    J Cell Sci. 1978 Apr;30:331-52 PMID: 348712
  3. Tubulin hooks as probes for microtubule polarity: an analysis of the method and an evaluation of data on microtubule polarity in the mitotic spindle.
    J Cell Biol. 1984 Feb;98(2):525-33 PMID: 6693493
  4. Striated flagellar roots: isolation and partial characterization of a calcium-modulated contractile organelle.
    J Cell Biol. 1984 Sep;99(3):962-70 PMID: 6381510
  5. Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5512-6 PMID: 3526331
  6. Diverse effects of beta-tubulin mutations on microtubule formation and function.
    J Cell Biol. 1988 Jun;106(6):1997-2010 PMID: 3290223
  7. The carboxy-terminal segment of the yeast alpha-factor receptor is a regulatory domain.
    Cell. 1988 Oct 21;55(2):221-34 PMID: 2844413
  8. Functions of microtubules in the Saccharomyces cerevisiae cell cycle.
    J Cell Biol. 1988 Oct;107(4):1409-26 PMID: 3049620
  9. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.
    Genetics. 1989 May;122(1):19-27 PMID: 2659436
  10. Mitosis.
    Science. 1989 Nov 3;246(4930):622-8 PMID: 2683078
  11. Astral microtubules are not required for anaphase B in Saccharomyces cerevisiae.
    J Cell Biol. 1992 Oct;119(2):379-88 PMID: 1400581
  12. Molecular cloning and centrosomal localization of human caltractin.
    Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11039-43 PMID: 8248209
  13. In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin.
    Cell. 1994 Feb 25;76(4):623-37 PMID: 8124706
  14. Cloning of a cDNA encoding human centrin, an EF-hand protein of centrosomes and mitotic spindle poles.
    J Cell Sci. 1994 Jan;107 ( Pt 1):9-16 PMID: 8175926
  15. STU1, a suppressor of a beta-tubulin mutation, encodes a novel and essential component of the yeast mitotic spindle.
    J Cell Biol. 1994 Dec;127(6 Pt 2):1973-84 PMID: 7806575
  16. Gamma-tubulin is required for the structure and function of the microtubule organizing centre in Drosophila neuroblasts.
    EMBO J. 1995 Jan 3;14(1):28-36 PMID: 7828594
  17. The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body.
    J Cell Biol. 1995 Mar;128(5):863-77 PMID: 7876310
  18. Analysis of the gamma-tubulin sequences: implications for the functional properties of gamma-tubulin.
    J Cell Sci. 1995 Jun;108 ( Pt 6):2123-30 PMID: 7673333
  19. gamma-tubulin is a minus end-specific microtubule binding protein.
    J Cell Biol. 1995 Oct;131(1):207-14 PMID: 7559777
  20. Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.
    Nature. 1995 Dec 7;378(6557):578-83 PMID: 8524390
  21. Microtubule nucleation by gamma-tubulin-containing rings in the centrosome.
    Nature. 1995 Dec 7;378(6557):638-40 PMID: 8524401
  22. A highly divergent gamma-tubulin gene is essential for cell growth and proper microtubule organization in Saccharomyces cerevisiae.
    J Cell Biol. 1995 Dec;131(6 Pt 2):1775-88 PMID: 8557744
  23. Chromosome instability mutants of Saccharomyces cerevisiae that are defective in microtubule-mediated processes.
    Mol Cell Biol. 1990 Jan;10(1):223-34 PMID: 2403635
  24. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.
    Curr Genet. 1989 Dec;16(5-6):339-46 PMID: 2692852
  25. Yeast mutants sensitive to antimicrotubule drugs define three genes that affect microtubule function.
    Genetics. 1990 Feb;124(2):251-62 PMID: 2407611
  26. Gamma-tubulin is a component of the spindle pole body that is essential for microtubule function in Aspergillus nidulans.
    Cell. 1990 Jun 29;61(7):1289-301 PMID: 2194669
  27. Components of the yeast spindle and spindle pole body.
    J Cell Biol. 1990 Nov;111(5 Pt 1):1913-27 PMID: 2229181
  28. Preparation of yeast cells for thin-section electron microscopy.
    Methods Enzymol. 1991;194:602-8 PMID: 2005811
  29. Gamma-tubulin is a highly conserved component of the centrosome.
    Cell. 1991 May 31;65(5):825-36 PMID: 1840506
  30. S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function.
    Cell. 1991 Aug 9;66(3):507-17 PMID: 1651171
  31. Feedback control of mitosis in budding yeast.
    Cell. 1991 Aug 9;66(3):519-31 PMID: 1651172
  32. CDC15, an essential cell cycle gene in Saccharomyces cerevisiae, encodes a protein kinase domain.
    Yeast. 1991 Apr;7(3):265-73 PMID: 1882551
  33. The fission yeast gamma-tubulin is essential for mitosis and is localized at microtubule organizing centers.
    J Cell Sci. 1991 Aug;99 ( Pt 4):693-700 PMID: 1770000
  34. Gamma-tubulin is a centrosomal protein required for cell cycle-dependent microtubule nucleation.
    Nature. 1992 Mar 5;356(6364):80-3 PMID: 1538786
  35. Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assembly.
    J Cell Biol. 1992 Jul;118(1):109-20 PMID: 1618897
  36. Kinesin-related proteins required for structural integrity of the mitotic spindle.
    Cell. 1992 Aug 7;70(3):451-8 PMID: 1643659
  37. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  38. Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiae.
    J Bacteriol. 1975 Oct;124(1):511-23 PMID: 1100612
  39. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  40. Sequential function of gene products relative to DNA synthesis in the yeast cell cycle.
    J Mol Biol. 1976 Jul 15;104(4):803-17 PMID: 785015
  41. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  42. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.134.2.429
Published
1996-07-00
Pages
429-41
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120879
Subset
IM
Databases
GENBANK
U14913
External Links
PubMed source DOI: 10.1083/jcb.134.2.429 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2