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PMID: 8722772 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The REC1 gene of Ustilago maydis, which encodes a 3'-->5' exonuclease, couples DNA repair and completion of DNA synthesis to a mitotic checkpoint.

Genetics ·Vol. 143 ·No. 1 ·1996-05-00 ·Pages 165-74

Onel K, Koff A, Bennett RL, Unrau P, Holloman WK

Abstract

Mutation in the REC1 gene of Ustilago maydis results in extreme sensitivity to killing by ultraviolet light. The lethality of the rec1-1 mutant was found to be partially suppressed if irradiated cells were held artificially in G2-phase by addition of a microtubule inhibitor. This mutant was also found to be sensitive to killing when DNA synthesis was inhibited by external means through addition of hydroxyurea or by genetic control in a temperature-sensitive mutant strain defective in DNA synthesis. Flow cytometric analysis of exponentially growing cultures indicated that wild-type cells accumulated in G2 after UV irradiation, while rec1-1 cells appeared to exit from G2 and accumulate in G1/S. Analysis of mRNA levels in synchronized cells indicated that the REC1 gene is periodically expressed with the cell cycle and reaches maximal levels at G1/S. The results are interpreted to mean that a G2-M checkpoint is disabled in the rec1-1 mutant. It is proposed that the REC1 gene product functions in a surveillance system operating during S-phase and G2 to find and repair stretches of DNA with compromised integrity and to communicate with the cell cycle apparatus.

MeSH Terms
Base Sequence Cell Cycle/radiation effects DNA Damage DNA Primers DNA Repair/radiation effects DNA Replication DNA, Fungal/biosynthesis Exodeoxyribonuclease V Exodeoxyribonucleases/biosynthesis,genetics Fungal Proteins/biosynthesis,genetics Kinetics Mitosis Molecular Sequence Data Mutagenesis, Site-Directed Polymerase Chain Reaction Time Factors Ultraviolet Rays Ustilago/cytology,genetics,physiology
Chemicals
DNA Primers DNA, Fungal Fungal Proteins Exodeoxyribonucleases Exodeoxyribonuclease V
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Onel K
Hearst Microbiology Research Center, Cornell University Medical College, New York, New York 10021, USA.
Koff A
Bennett R L
Unrau P
Holloman W K
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/143.1.165
Published
1996-05-00
Pages
165-74
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1207251
Subset
IM
Grants
NIGMS NIH HHS · GM-42482 · United States
External Links
PubMed source DOI: 10.1093/genetics/143.1.165 PMC Full Text View journal details
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