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PMID: 8858167 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor.

The Journal of cell biology ·Vol. 135 ·No. 1 ·1996-10-00 ·Pages 111-21

Erdmann R, Blobel G

Abstract

We have identified an S. cerevisiae integral peroxisomal membrane protein of M of 42,705 (Pex13p) that is a component of the peroxisomal protein import apparatus. Pex13p's most striking feature is an src homology 3 (SH3) domain that interacts directly with yeast Pex5p (former Pas10p), the recognition factor for the COOH-terminal tripeptide signal sequence (PTS1), but not with Pex7p (former Pas7p), the recognition factor for the NH2-terminal nonapeptide signal (PTS2) of peroxisomal matrix proteins. Hence, Pex13p serves as peroxisomal membrane receptor for at least one of the two peroxisomal signal recognition factors. Cells deficient in Pex13p are unable to import peroxisomal matrix proteins containing PTS1 and, surprisingly, also those containing PTS2. Pex13p deficient cells retain membranes containing the peroxisomal membrane protein Pex11p (former Pmp27p), consistent with the existence of independent pathways for the integration of peroxisomal membrane proteins and for the translocation of peroxisomal matrix proteins.

MeSH Terms
Acetyl-CoA C-Acetyltransferase/analysis Amino Acid Sequence Biological Transport Catalase/analysis Cloning, Molecular Fumarate Hydratase/analysis Fungal Proteins/analysis Genes, Fungal/genetics Intracellular Membranes/chemistry Membrane Proteins/analysis,chemistry,genetics,isolation & purification,metabolism,physiology Microbodies/chemistry,metabolism Molecular Sequence Data Oleic Acid Peroxins Peroxisomal Targeting Signal 2 Receptor Peroxisome-Targeting Signal 1 Receptor Receptors, Cytoplasmic and Nuclear/metabolism Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Analysis src Homology Domains
Chemicals
Fungal Proteins Membrane Proteins PEX11 protein, S cerevisiae PEX11A protein, human PEX11B protein, human PEX13 protein, S cerevisiae Peroxins Peroxisomal Targeting Signal 2 Receptor Peroxisome-Targeting Signal 1 Receptor Receptors, Cytoplasmic and Nuclear Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Oleic Acid Catalase Acetyl-CoA C-Acetyltransferase Fumarate Hydratase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Erdmann R
Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, New York 10021, USA. [email protected]
Blobel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.135.1.111
Email
[email protected]
Published
1996-10-00
Pages
111-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2121012
Subset
IM
Databases
GENBANK
S51436, U20864
External Links
PubMed source DOI: 10.1083/jcb.135.1.111 PMC Full Text View journal details
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