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PMID: 8995695 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Macrophage tropism of human immunodeficiency virus type 1 and utilization of the CC-CKR5 coreceptor.

Journal of virology ·Vol. 71 ·No. 2 ·1997-02-00 ·Pages 1657-61

Cheng-Mayer C, Liu R, Landau NR, Stamatatos L

Abstract

The recent identification of the CC-CKR5 beta chemokine receptor as a major cofactor for entry of macrophage-tropic isolates of human immunodeficiency virus type 1 (HIV-1) raises the question of whether macrophage tropism is determined by utilization of this chemokine receptor. We observe that in addition to macrophage-tropic isolates of clades A, B, and E, macrophage-tropic isolates of clade F also utilize the CC-CKR5 molecule for entry. However, using single-round replication-competent reporter viruses carrying the envelope genes of T-cell line-tropic or macrophage-tropic phenotypic recombinant and mutant HIV-1 strains in infection of stable cell lines that coexpress the CD4 and chemokine receptors, we were unable to establish a strict correlation between macrophage tropism and utilization of the CC-CKR5 chemokine receptor. This latter finding suggests that a cofactor other than CC-CKR5 serves to determine entry into primary macrophages.

MeSH Terms
Amino Acid Sequence HIV Infections/virology HIV-1/physiology Humans Macrophages/virology Molecular Sequence Data Receptors, CCR5 Receptors, Cytokine Receptors, HIV Virus Replication
Chemicals
Receptors, CCR5 Receptors, Cytokine Receptors, HIV
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Cheng-Mayer C
Aaron Diamond AIDS Research Center, The Rockefeller University, New York, New York 10021-6399, USA.
Liu R
Landau N R
Stamatatos L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.2.1657-1661.1997
Published
1997-02-00
Pages
1657-61
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC191226
Subset
IM
Grants
NCI NIH HHS · R01 CA72149 · United States
NCI NIH HHS · R01 CA72822 · United States
NIAID NIH HHS · R29 AI36057 · United States
External Links
PubMed source DOI: 10.1128/JVI.71.2.1657-1661.1997 PMC Full Text View journal details
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