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PMID: 9224601 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mechanisms of developmental regulation in Trypanosoma brucei: a polypyrimidine tract in the 3'-untranslated region of a surface protein mRNA affects RNA abundance and translation.

Nucleic acids research ·Vol. 25 ·No. 15 ·1997-08-01 ·Pages 3017-26

Hotz HR, Hartmann C, Huober K, Hug M, Clayton C

Abstract

Salivarian trypanosomes are extracellular parasites of mammals that are transmitted by tsetse flies. The procyclic acidic repetitive proteins (PARPs) are the major surface glycoproteins of the form of Trypanosoma brucei that replicates in the fly. The abundance of PARP mRNA and protein is very strongly regulated, mostly at the post-transcriptional level. The 3'-untranslated regions of two PARP genes are of similar lengths, but are dissimilar in sequence apart from a 16mer stem-loop that stimulates translation and a 26mer polypyrimidine tract. Addition of either of these PARP 3'-untranslated regions immediately downstream of a reporter gene resulted in developmental regulation mimicking that of PARP. We show that the PARP 3'-UTR reduces RNA stability and translation in bloodstream forms and that the 26mer polypyrimidine tract is necessary for both effects.

MeSH Terms
Animals Base Sequence Gene Expression Regulation, Developmental Membrane Glycoproteins/genetics Molecular Sequence Data Mutagenesis Protein Biosynthesis Protozoan Proteins/genetics Pyrimidines RNA Splicing RNA, Messenger/metabolism RNA, Protozoan/metabolism Trypanosoma brucei brucei/genetics
Chemicals
Membrane Glycoproteins Protozoan Proteins Pyrimidines RNA, Messenger RNA, Protozoan procyclic acidic repetitive protein, Trypanosoma
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hotz H R
Zentrum für Molekulare Biologie, Universität Heidelberg, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany.
Hartmann C
Huober K
Hug M
Clayton C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.15.3017
Published
1997-08-01
Pages
3017-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146859
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/25.15.3017 PMC Full Text View journal details
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