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PMID: 9356502 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Yersinia enterocolitica induces apoptosis in macrophages by a process requiring functional type III secretion and translocation mechanisms and involving YopP, presumably acting as an effector protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 94 ·No. 23 ·1997-11-11 ·Pages 12638-43

Mills SD, Boland A, Sory MP, van der Smissen P, Kerbourch C, Finlay BB, Cornelis GR

Abstract

Yersiniae, causative agents of plague and gastrointestinal diseases, secrete and translocate Yop effector proteins into the cytosol of macrophages, leading to disruption of host defense mechanisms. It is shown in this report that Yersinia enterocolitica induces apoptosis in macrophages and that this effect depends on YopP. Functional secretion and translocation mechanisms are required for YopP to act, strongly suggesting that this protein exerts its effect intracellularly, after translocation into the macrophages. YopP shows a high level of sequence similarity with AvrRxv, an avirulence protein from Xanthomonas campestris, a plant pathogen that induces programmed cell death in plant cells. This indicates possible similarities between the strategies used by pathogenic bacteria to elicit programmed cell death in both plant and animal hosts.

MeSH Terms
Amino Acid Sequence Animals Apoptosis Bacterial Proteins/genetics,metabolism Cell Line Macrophages/microbiology,pathology Mice Molecular Sequence Data Yersinia Infections/metabolism,pathology Yersinia enterocolitica
Chemicals
Bacterial Proteins YopP protein, Yersinia
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Mills S D
Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia V6T 1Z3 Canada.
Boland A
Sory M P
van der Smissen P
Kerbourch C
Finlay B B
Cornelis G R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.94.23.12638
Published
1997-11-11
Pages
12638-43
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC25065
Subset
IM
Databases
GENBANK
AF023202
External Links
PubMed source DOI: 10.1073/pnas.94.23.12638 PMC Full Text View journal details
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