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PMID: 9382872 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body.

The Journal of cell biology ·Vol. 139 ·No. 5 ·1997-12-01 ·Pages 1271-80

Wang PJ, Huffaker TC

Abstract

Previously we isolated tub2-423, a cold-sensitive allele of the Saccharomyces cerevisiae gene encoding beta-tubulin that confers a defect in mitotic spindle function. In an attempt to identify additional proteins that are important for spindle function, we screened for suppressors of the cold sensitivity of tub2-423 and obtained two alleles of a novel gene, STU2. STU2 is an essential gene and encodes a protein whose sequence is similar to proteins identified in a variety of organisms. Stu2p localizes primarily to the spindle pole body (SPB) and to a lesser extent along spindle microtubules. Localization to the SPB is not dependent on the presence of microtubules, indicating that Stu2p is an integral component of the SPB. Stu2p also binds microtubules in vitro. We have localized the microtubule-binding domain of Stu2p to a highly basic 100-amino acid region. This region contains two imperfect repeats; both repeats appear to contribute to microtubule binding to similar extents. These results suggest that Stu2p may play a role in the attachment, organization, and/or dynamics of microtubule ends at the SPB.

MeSH Terms
Alleles Amino Acid Sequence Binding Sites Centrosome/chemistry,metabolism Cloning, Molecular Fungal Proteins/genetics,isolation & purification,metabolism Genes, Fungal Genes, Lethal Green Fluorescent Proteins Luminescent Proteins/genetics,isolation & purification Microtubule-Associated Proteins/genetics,isolation & purification,metabolism Microtubules/metabolism Molecular Sequence Data Protein Binding Recombinant Fusion Proteins/isolation & purification Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Spindle Apparatus/chemistry,metabolism Suppression, Genetic
Chemicals
Fungal Proteins Luminescent Proteins Microtubule-Associated Proteins Recombinant Fusion Proteins STU2 protein, S cerevisiae Saccharomyces cerevisiae Proteins Green Fluorescent Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang P J
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853, USA.
Huffaker T C
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.139.5.1271
Published
1997-12-01
Pages
1271-80
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2140218
Subset
IM
Grants
NIGMS NIH HHS · R01 GM040479 · United States
NIGMS NIH HHS · GM40479 · United States
Databases
GENBANK
U35247
External Links
PubMed source DOI: 10.1083/jcb.139.5.1271 PMC Full Text View journal details
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