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PMID: 9383049 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A role for REV3 in mutagenesis during double-strand break repair in Saccharomyces cerevisiae.

Genetics ·Vol. 147 ·No. 3 ·1997-11-00 ·Pages 1017-24

Holbeck SL, Strathern JN

Abstract

Recombinational repair of double-strand breaks (DSBs), traditionally believed to be an error-free DNA repair pathway, was recently shown to increase the frequency of mutations in a nearby interval. The reversion rate of trp1 alleles (either nonsense or frameshift mutations) near an HO-endonuclease cleavage site is increased at least 100-fold among cells that have experienced an HO-mediated DSB. We report here that in strains deleted for rev3 this DSB-associated reversion of a nonsense mutation was greatly decreased. Thus REV3, which encodes a subunit of the translesion DNA polymerase zeta, was responsible for the majority of these base substitution errors near a DSB. However, rev3 strains showed no decrease in HO-stimulated recombination, implying that another DNA polymerase also functioned in recombinational repair of a DSB. Reversion of trp1 frameshift alleles near a DSB was not reduced in rev3 strains, indicating that another polymerase could act during DSB repair to make these frameshift errors. Analysis of spontaneous reversion in haploid strains suggested that Rev3p had a greater role in making point mutations than in frameshift mutations.

MeSH Terms
Aldose-Ketose Isomerases DNA Repair DNA-Directed DNA Polymerase/physiology Deoxyribonucleases, Type II Site-Specific/metabolism Frameshift Mutation Fungal Proteins/genetics,physiology Mutagenesis Point Mutation Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins
Chemicals
Fungal Proteins Saccharomyces cerevisiae Proteins DNA-Directed DNA Polymerase REV3 protein, S cerevisiae HO protein, S cerevisiae SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific Aldose-Ketose Isomerases TRP1 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Holbeck S L
National Cancer Institute-Frederick Cancer Research and Development Center, ABL-Basic Research Program, Maryland 21702-1201, USA.
Strathern J N
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/147.3.1017
Published
1997-11-00
Pages
1017-24
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1208230
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/147.3.1017 PMC Full Text View journal details
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