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PMID: 9393708 Published · ppublish English Journal Article

Physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome.

Journal of bacteriology ·Vol. 179 ·No. 23 ·1997-12-00 ·Pages 7426-34

Cornillot E, Croux C, Soucaille P

Abstract

A physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome was constructed. The macrorestriction map for CeuI, EagI, and SstII was created by ordering the 38 restriction sites by one- and two-dimensional pulsed-field gel electrophoresis (PFGE) and by using an original strategy based on the CeuI enzyme and indirect end labelling by hybridization on both sides of the CeuI sites with rrs (16S RNA) and 3' rrl (23S RNA) probes. The circular chromosome was estimated to be 4.15 Mb in size, and the average resolution of the physical map is 110 kb. The chromosome contains 11 rrn loci, which are localized on 44% of the chromosome in a divergent transcriptional orientation regarding the presumed location of the replication origin. In addition to these 11 rrn operons, a total of 40 identified genes were mapped by hybridization experiments with genes from C. acetobutylicum and from various other clostridia as probes. The genetic map of C. acetobutylicum was compared to that of the three other endospore-forming bacteria characterized so far: Bacillus subtilis, Clostridium beijerinckii, and Clostridium perfringens. Parodoxically, the chromosomal backbone of C. acetobutylicum showed more similarity to that of B. subtilis than to those of the clostridia.

MeSH Terms
Chromosomes, Bacterial Clostridium/genetics DNA, Bacterial/genetics DNA, Ribosomal/genetics Endodeoxyribonucleases Gene Dosage Gene Expression Genes, Bacterial Genome, Bacterial Nucleic Acid Hybridization Oligonucleotide Probes Operon Restriction Mapping
Chemicals
DNA, Bacterial DNA, Ribosomal Oligonucleotide Probes Endodeoxyribonucleases endodeoxyribonuclease I-CeuI
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cornillot E
INSA, Centre de Bioingénierie G. Durand, UMR CNRS 5504, Laboratoire associé INRA, Toulouse, France.
Croux C
Soucaille P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.23.7426-7434.1997
Published
1997-12-00
Pages
7426-34
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179694
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.179.23.7426-7434.1997 PMC Full Text View journal details
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