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PMID: 1708377 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical map of the chromosome of Lactococcus lactis subsp. lactis DL11 and localization of six putative rRNA operons.

Journal of bacteriology ·Vol. 173 ·No. 9 ·1991-05-00 ·Pages 2768-75

Tulloch DL, Finch LR, Hillier AJ, Davidson BE

Abstract

A physical map of the chromosome of Lactococcus lactis subsp. lactis DL11 was constructed by using the contour-clamped homogeneous electric field mode of pulsed-field gel electrophoresis in one- and two-dimensional separations to analyze restriction digests of high-molecular-weight genomic DNA. The map, which shows all the observed NotI and SmaI sites (six and 21, respectively) and 8 of approximately 30 SalI sites, is circular and yields a total size of 2.58 megabase pairs for the L. lactis subsp. lactis DL11 chromosome. By using rDNA from Mycoplasma capricolum to probe Southern blots of pulsed-and fixed-field digestion patterns, six putative rRNA operons were identified in L. lactis subsp. lactis DL11 and placed on the map of the chromosome. Five of these loci are clustered in a region representing only 20% of the chromosome. The presence of a SmaI site in each of the putative operons allowed the direction of transcription of each operon to be deduced.

MeSH Terms
Blotting, Southern Electrophoresis, Gel, Two-Dimensional Genes, Bacterial Lactococcus lactis/genetics Operon/genetics Plasmids Polymerase Chain Reaction Polymorphism, Restriction Fragment Length RNA/genetics RNA Probes Restriction Mapping Transcription, Genetic
Chemicals
RNA Probes RNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tulloch D L
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia.
Finch L R
Hillier A J
Davidson B E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.9.2768-2775.1991
Published
1991-05-00
Pages
2768-75
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207856
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.9.2768-2775.1991 PMC Full Text View journal details
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