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PMID: 9484470 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparative analysis of the nucleosomal structure of rye, wheat and their relatives.

Plant molecular biology ·Vol. 36 ·No. 1 ·1998-01-00 ·Pages 149-61

Vershinin AV, Heslop-Harrison JS

Abstract

Analysis of the structure of chromatin in cereal species using micrococcal nuclease (MNase) cleavage showed nucleosomal organization and a ladder with typical nucleosomal spacing of 175-185 bp. Probing with a set of DNA probes localized in the authentic telomeres, subtelomeric regions and bulk chromatin revealed that these chromosomal regions have nucleosomal organization but differ in size of nucleosomes and rate of cleavage between both species and regions. Chromatin from Secale and Dasypyrum cleaved more quickly than that from wheat and barley, perhaps because of their higher content of repetitive sequences with hairpin structures accessible to MNase cleavage. In all species, the telomeric chromatin showed more rapid cleavage kinetics and a shorter nucleosome length (160 bp spacing) than bulk chromatin. Rye telomeric repeat arrays were shortest, ranging from 8 kb to 50 kb while those of wheat ranged from 15 kb up to 175 kb. A gradient of sensitivity to MNase was detected along rye chromosomes. The rye-specific subtelomeric sequences pSc200 and pSc250 have nucleosomes of two lengths, those of the telomeric and of bulk nucleosomes, indicating that the telomeric structure may extended into the chromosomes. More proximal sequences common to rye and wheat, the short tandem-repeat pSc119.2 and rDNA sequence pTa71, showed longer nucleosomal sizes characteristic of bulk chromatin in both species. A strictly defined spacing arrangement (phasing) of nucleosomes was demonstrated along arrays of tandem repeats with different monomer lengths (118, 350 and 550 bp) by combining MNase and restriction enzyme digestion.

MeSH Terms
Base Composition Chromatin/chemistry,metabolism DNA Probes DNA, Plant/chemistry Nucleosomes/genetics,ultrastructure Secale/genetics,ultrastructure Species Specificity Telomere/genetics,ultrastructure Triticum/genetics,ultrastructure
Chemicals
Chromatin DNA Probes DNA, Plant Nucleosomes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vershinin A V
Department of Cell Biology, John Innes Centre, Norwich, UK.
Heslop-Harrison J S
References (47)
47 references, click to expand
  1. Simultaneous discrimination of the three genomes in hexaploid wheat by multicolor fluorescence in situ hybridization using total genomic and highly repeated DNA probes.
    Genome. 1993 Jun;36(3):489-94 PMID: 18470003
  2. Curved DNA.
    CRC Crit Rev Biochem. 1985;19(2):89-106 PMID: 3905255
  3. Structural specificities of five commonly used DNA nucleases.
    J Mol Biol. 1984 Jul 15;176(4):535-57 PMID: 6086935
  4. Nuclease hypersensitive sites in chromatin.
    Annu Rev Biochem. 1988;57:159-97 PMID: 3052270
  5. A family of related sequences associated with (TTTAGGG)n repeats are located in the interstitial regions of wheat chromosomes.
    Mol Gen Genet. 1994 Nov 1;245(3):349-54 PMID: 7816045
  6. A unified model of eukaryotic chromosomes.
    Cytometry. 1990;11(1):8-25 PMID: 2307065
  7. High sequence specificity of micrococcal nuclease.
    Nucleic Acids Res. 1981 Jun 25;9(12):2659-73 PMID: 6269057
  8. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.
    J Mol Biol. 1962 Jun;4:430-43 PMID: 14498379
  9. Cloning and characterization of ribosomal RNA genes from wheat and barley.
    Nucleic Acids Res. 1979 Dec 11;7(7):1869-85 PMID: 537913
  10. Unusual chromatin in human telomeres.
    Mol Cell Biol. 1994 Sep;14(9):5777-85 PMID: 8065312
  11. Molecular diversification of tandemly organized DNA sequences and heterochromatic chromosome regions in some Triticeae species.
    Chromosome Res. 1996 Nov;4(7):517-25 PMID: 8939363
  12. Deviant nucleosomes: the functional specialization of chromatin.
    Trends Genet. 1996 Feb;12(2):58-62 PMID: 8851972
  13. The isolation of high molecular weight DNA from wheat, barley and rye for analysis by pulse-field gel electrophoresis.
    Plant Mol Biol. 1990 May;14(5):881-8 PMID: 1966389
  14. Genetic and physical mapping of barley telomeres.
    Mol Gen Genet. 1993 Apr;238(1-2):294-303 PMID: 8479435
  15. Characterization of a new family of tobacco highly repetitive DNA, GRS, specific for the Nicotiana tomentosiformis genomic component.
    Chromosome Res. 1995 Jun;3(4):245-54 PMID: 7606363
  16. Molecular drive: a cohesive mode of species evolution.
    Nature. 1982 Sep 9;299(5879):111-7 PMID: 7110332
  17. Structure of nucleosomes and organization of internucleosomal DNA in chromatin.
    J Mol Biol. 1990 Apr 5;212(3):495-511 PMID: 2325131
  18. DNA associated with nucleosomes in plants.
    Nucleic Acids Res. 1977 Oct;4(10):3617-26 PMID: 928070
  19. Analysis of nucleosomal deoxyribonucleic acid in a higher plant.
    Biochem J. 1977 Apr 1;163(1):141-4 PMID: 869912
  20. Nucleosomal organization of telomere-specific chromatin in rat.
    Cell. 1993 May 21;73(4):775-87 PMID: 8500170
  21. Chromatin structure of the telomeric region and 3'-nontranscribed spacer of Tetrahymena ribosomal RNA genes.
    J Biol Chem. 1986 Jan 5;261(1):363-9 PMID: 3001053
  22. Repetitive DNA and chromosome evolution in plants.
    Philos Trans R Soc Lond B Biol Sci. 1986 Jan 29;312(1154):227-42 PMID: 2870519
  23. Telomeres: beginning to understand the end.
    Science. 1995 Dec 8;270(5242):1601-7 PMID: 7502069
  24. Organization of telomeric and subtelomeric chromatin in the higher plant Nicotiana tabacum.
    Mol Gen Genet. 1995 Jun 10;247(5):633-8 PMID: 7603443
  25. Position effect variegation in Drosophila is associated with an altered chromatin structure.
    Genes Dev. 1995 May 15;9(10):1263-77 PMID: 7758950
  26. Nucleosome positioning: occurrence, mechanisms, and functional consequences.
    Prog Nucleic Acid Res Mol Biol. 1991;40:143-84 PMID: 2031082
  27. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity.
    EMBO J. 1992 May;11(5):1921-9 PMID: 1582420
  28. Curvature of mouse satellite DNA and condensation of heterochromatin.
    Cell. 1987 Sep 25;50(7):1101-8 PMID: 2441880
  29. Isolation of a higher eukaryotic telomere from Arabidopsis thaliana.
    Cell. 1988 Apr 8;53(1):127-36 PMID: 3349525
  30. Structure of the human centromere at metaphase.
    Trends Biochem Sci. 1990 May;15(5):181-5 PMID: 2193435
  31. Barley telomeres shorten during differentiation but grow in callus culture.
    Proc Natl Acad Sci U S A. 1995 Oct 10;92(21):9555-9 PMID: 11607583
  32. The large-scale genomic organization of repetitive DNA families at the telomeres of rye chromosomes.
    Plant Cell. 1995 Nov;7(11):1823-33 PMID: 8535136
  33. DNA Comparisons among Barley, Oats, Rye, and Wheat.
    Genetics. 1970 Aug;65(4):545-65 PMID: 17248502
  34. The evolutionary dynamics of repetitive DNA in eukaryotes.
    Nature. 1994 Sep 15;371(6494):215-20 PMID: 8078581
  35. Evidence for nucleosomal phasing and a novel protein specifically binding to cucumber satellite DNA.
    Z Naturforsch C. 1994 Jan-Feb;49(1-2):79-86 PMID: 8148012
  36. Centromeres and kinetochores: integrated domains on eukaryotic chromosomes.
    Curr Opin Cell Biol. 1990 Jun;2(3):446-52 PMID: 2198897
  37. Characterization of relic DNA from barley genome.
    Theor Appl Genet. 1990 Sep;80(3):374-80 PMID: 24220972
  38. Purification of the centromere-specific protein CENP-A and demonstration that it is a distinctive histone.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3734-8 PMID: 2023923
  39. Nuclear dna amounts in angiosperms.
    Philos Trans R Soc Lond B Biol Sci. 1976 May 27;274(933):227-74 PMID: 6977
  40. Properties of condensed chromatin in barley nuclei.
    Planta. 1980 Jun;149(1):69-77 PMID: 24306195
  41. Mobile nucleosomes--a general behavior.
    EMBO J. 1992 Aug;11(8):2951-9 PMID: 1639066
  42. Comparative RFLP maps of the homoeologous group-2 chromosomes of wheat, rye and barley.
    Theor Appl Genet. 1993 Feb;85(6-7):784-92 PMID: 24196051
  43. Role of chromosome territories in the functional compartmentalization of the cell nucleus.
    Cold Spring Harb Symp Quant Biol. 1993;58:777-92 PMID: 7525149
  44. New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat.
    Genome. 1990 Oct;33(5):635-40 PMID: 2262137
  45. Eight different highly specific nucleosome phases on alpha-satellite DNA in the African green monkey.
    Nucleic Acids Res. 1983 Jul 11;11(13):4287-306 PMID: 6306583
  46. Saccharomyces telomeres assume a non-nucleosomal chromatin structure.
    Genes Dev. 1992 Feb;6(2):197-210 PMID: 1737616
  47. The chromatin structure of centromeres from fission yeast: differentiation of the central core that correlates with function.
    J Cell Biol. 1991 Jan;112(2):191-201 PMID: 1988457
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1023/a:1005912822671
Published
1998-01-00
Pages
149-61
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1023/a:1005912822671 View journal details
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