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PMID: 9679141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The cytosolic DnaJ-like protein djp1p is involved specifically in peroxisomal protein import.

The Journal of cell biology ·Vol. 142 ·No. 2 ·1998-07-27 ·Pages 421-34

Hettema EH, Ruigrok CC, Koerkamp MG, van den Berg M, Tabak HF, Distel B, Braakman I

Abstract

The Saccharomyces cerevisiae DJP1 gene encodes a cytosolic protein homologous to Escherichia coli DnaJ. DnaJ homologues act in conjunction with molecular chaperones of the Hsp70 protein family in a variety of cellular processes. Cells with a DJP1 gene deletion are viable and exhibit a novel phenotype among cytosolic J-protein mutants in that they have a specific impairment of only one organelle, the peroxisome. The phenotype was also unique among peroxisome assembly mutants: peroxisomal matrix proteins were mislocalized to the cytoplasm to a varying extent, and peroxisomal structures failed to grow to full size and exhibited a broad range of buoyant densities. Import of marker proteins for the endoplasmic reticulum, nucleus, and mitochondria was normal. Furthermore, the metabolic adaptation to a change in carbon source, a complex multistep process, was unaffected in a DJP1 gene deletion mutant. We conclude that Djp1p is specifically required for peroxisomal protein import.

MeSH Terms
Amino Acid Sequence Base Sequence Biological Transport, Active Cloning, Molecular Cytosol/metabolism DNA Primers/genetics Escherichia coli/genetics,metabolism Escherichia coli Proteins Fungal Proteins/genetics,metabolism Gene Deletion Gene Expression Genes, Fungal Green Fluorescent Proteins HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins/metabolism Heat-Shock Proteins/genetics,metabolism Luminescent Proteins/genetics,metabolism Microbodies/metabolism Microscopy, Immunoelectron Molecular Sequence Data Phenotype Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Sequence Homology, Amino Acid
Chemicals
DNA Primers DnaJ protein, E coli Escherichia coli Proteins Fungal Proteins HSP40 Heat-Shock Proteins HSP70 Heat-Shock Proteins Heat-Shock Proteins Luminescent Proteins Green Fluorescent Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Hettema E H
Department of Biochemistry, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands.
Ruigrok C C
Koerkamp M G
van den Berg M
Tabak H F
Distel B
Braakman I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.142.2.421
Published
1998-07-27
Pages
421-34
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133058
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.142.2.421 PMC Full Text View journal details
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