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PMID: 8195287 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Involvement of 70-kD heat-shock proteins in peroxisomal import.

The Journal of cell biology ·Vol. 125 ·No. 5 ·1994-06-00 ·Pages 1037-46

Walton PA, Wendland M, Subramani S, Rachubinski RA, Welch WJ

Abstract

This report describes the involvement of 70-kD heat-shock proteins (hsp70) in the import of proteins into mammalian peroxisomes. Employing a microinjection-based assay (Walton, P. A., S. J. Gould, J. R. Feramisco, and S. Subramani. 1992. Mol. Cell Biol. 12:531-541), we demonstrate that proteins of the hsp70 family were associated with proteins being imported into the peroxisomal matrix. Import of peroxisomal proteins could be inhibited by coinjection of antibodies directed against the constitutive hsp70 proteins (hsp73). In a permeabilized-cell assay (Wendland and Subramani. 1993. J. Cell Biol. 120:675-685), antibodies directed against hsp70 proteins were shown to inhibit peroxisomal protein import. Inhibition could be overcome by the addition of exogenous hsp70 proteins. Purified rat liver peroxisomes were shown to have associated hsp70 proteins. The amount of associated hsp70 was increased under conditions of peroxisomal proliferation. Furthermore, proteinase protection assays indicated that the hsp70 molecules were located on the outside of the peroxisomal membrane. Finally, the process of heat-shocking cells resulted in a considerable delay in the import of peroxisomal proteins. Taken together, these results indicate that heat-shock proteins of the cytoplasmic hsp70 family are involved in the import of peroxisomal proteins.

MeSH Terms
Animals Biological Transport Blotting, Western Cell Line Endopeptidase K Fluorescent Antibody Technique Heat-Shock Proteins/chemistry,metabolism Hot Temperature Humans Liver/metabolism Microbodies/metabolism Rats Serine Endopeptidases/pharmacology
Chemicals
Heat-Shock Proteins Serine Endopeptidases Endopeptidase K
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Walton P A
Department of Anatomy and Cell Biology, McGill University, Montreal, Canada.
Wendland M
Subramani S
Rachubinski R A
Welch W J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.125.5.1037
Published
1994-06-00
Pages
1037-46
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120043
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.125.5.1037 PMC Full Text View journal details
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