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PMID: 9748444 Published · ppublish English Journal Article

Formation of single-stranded DNA during DNA transformation of Neisseria gonorrhoeae.

Journal of bacteriology ·Vol. 180 ·No. 19 ·1998-10-00 ·Pages 5117-22

Chaussee MS, Hill SA

Abstract

Neisseria gonorrhoeae is naturally competent for DNA transformation. In contrast to other natural prokaryotic DNA transformation systems, single-stranded donor DNA (ssDNA) has not previously been detected during transformation of N. gonorrhoeae. We have reassessed the physical nature of gonococcal transforming DNA by using a sensitive nondenaturing native blotting technique that detects ssDNA. Consistent with previous analyses, we found that the majority of donor DNA remained in the double-stranded form, and only plasmid DNAs that carried the genus-specific DNA uptake sequence were sequestered in a DNase I-resistant state. However, when the DNA was examined under native conditions, S1 nuclease-sensitive ssDNA was identified in all strains tested except for those bacteria that carried the dud-1 mutation. Surprisingly, ssDNA was also found during transformation of N. gonorrhoeae comA mutants, which suggested that ssDNA was initially formed within the periplasm.

MeSH Terms
Bacterial Proteins/genetics,physiology Blotting, Southern/methods DNA, Bacterial/biosynthesis,chemistry DNA, Single-Stranded/biosynthesis,chemistry DNA-Binding Proteins/genetics,physiology Mutation Neisseria gonorrhoeae/genetics,metabolism Nucleic Acid Conformation Plasmids/genetics Sensitivity and Specificity Transformation, Bacterial/genetics
Chemicals
Bacterial Proteins ComA protein, Bacteria DNA, Bacterial DNA, Single-Stranded DNA-Binding Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chaussee M S
Laboratory of Microbial Structure and Function, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840, USA [email protected]
Hill S A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.19.5117-5122.1998
Email
[email protected]
Published
1998-10-00
Pages
5117-22
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC107547
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.180.19.5117-5122.1998 PMC Full Text View journal details
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