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PMID: 9799243 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2.

The EMBO journal ·Vol. 17 ·No. 21 ·1998-11-02 ·Pages 6359-67

Xiao SH, Manley JL

Abstract

SR proteins are a conserved family of splicing factors that function in both constitutive and activated splicing. We reported previously that phosphorylation of the SR protein ASF/SF2 enhances its interaction with the U1 snRNP-specific 70K protein and is required for the protein to function in splicing, while other studies have provided evidence that subsequent dephosphorylation can also be required for SR protein function, at least in constitutive splicing. We now show that the phosphorylation status of ASF/SF2 can differentially affect several properties of the protein. In keeping with a dynamic cycle of phosphorylation-dephosphorylation during splicing, ASF/SF2 phosphorylation was found to affect interaction with several putative protein targets in different ways: positively, negatively or not at all. Extending these results, we also show that, in contrast to constitutive splicing, dephosphorylation is not required for ASF/SF2 to function as a splicing activator. We discuss these results with respect to the differential protein-protein interactions that must occur during constitutive and activated splicing.

MeSH Terms
Adenosine Triphosphate/analogs & derivatives,metabolism Animals Cell Extracts DNA-Binding Proteins/genetics Drosophila/metabolism Drosophila Proteins Gene Products, tat/genetics HeLa Cells Humans Insect Proteins/genetics Nuclear Proteins/metabolism Phosphoproteins/metabolism Phosphorylation Protein Binding RNA Precursors/genetics RNA Splicing/genetics RNA, Viral/genetics RNA-Binding Proteins/metabolism Recombinant Fusion Proteins/genetics Ribonucleoproteins/metabolism Serine-Arginine Splicing Factors Splicing Factor U2AF
Chemicals
Cell Extracts DNA-Binding Proteins DSX protein, Drosophila Drosophila Proteins Gene Products, tat Insect Proteins Nuclear Proteins Phosphoproteins RNA Precursors RNA, Viral RNA-Binding Proteins Recombinant Fusion Proteins Ribonucleoproteins Splicing Factor U2AF U2AF2 protein, human Serine-Arginine Splicing Factors adenosine 5'-O-(3-thiotriphosphate) Adenosine Triphosphate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Xiao S H
Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Manley J L
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.21.6359
Published
1998-11-02
Pages
6359-67
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170960
Subset
IM
Grants
NIGMS NIH HHS · GM48259 · United States
External Links
PubMed source DOI: 10.1093/emboj/17.21.6359 PMC Full Text View journal details
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