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PMID: 9847348 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional coupling between replication and packaging of poliovirus replicon RNA.

Journal of virology ·Vol. 73 ·No. 1 ·1999-01-00 ·Pages 427-35

Nugent CI, Johnson KL, Sarnow P, Kirkegaard K

Abstract

Poliovirus RNA genomes that contained deletions in the capsid-coding regions were synthesized in monkey kidney cells that constitutively expressed T7 RNA polymerase. These replication-competent subgenomic RNAs, or replicons (G. Kaplan and V. R. Racaniello, J. Virol. 62:1687-1696, 1988), were encapsidated in trans by superinfecting polioviruses. When superinfecting poliovirus resistant to the antiviral compound guanidine was used, the RNA replication of the replicon RNAs could be inhibited independently of the RNA replication of the guanidine-resistant helper virus. Inhibiting the replication of the replicon RNA also profoundly inhibited its trans-encapsidation, even though the capsid proteins present in the cells could efficiently encapsidate the helper virus. The observed coupling between RNA replication and RNA packaging could account for the specificity of poliovirus RNA packaging in infected cells and the observed effects of mutations in the coding regions of nonstructural proteins on virion morphogenesis. It is suggested that this coupling results from direct interactions between the RNA replication machinery and the capsid proteins. The coupling of RNA packaging to RNA replication and of RNA replication to translation (J. E. Novak and K. Kirkegaard, Genes Dev. 8:1726-1737, 1994) could serve as mechanisms for late proofreading of poliovirus RNA, allowing only those RNA genomes capable of translating a full complement of functional RNA replication proteins to be propagated.

MeSH Terms
Base Sequence Guanidine/pharmacology HeLa Cells Humans Molecular Sequence Data Poliovirus/genetics,physiology RNA, Viral/biosynthesis Replicon Virus Assembly Virus Replication
Chemicals
RNA, Viral Guanidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nugent C I
Department of Molecular, Cellular and Developmental Biology and Howard Hughes Medical Institute, University of Colorado, Boulder, Colorado 80309, USA.
Johnson K L
Sarnow P
Kirkegaard K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.73.1.427-435.1999
Published
1999-01-00
Pages
427-35
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC103849
Subset
IM
Grants
NIAID NIH HHS · AI25105 · United States
NIAID NIH HHS · AI25166 · United States
External Links
PubMed source DOI: 10.1128/JVI.73.1.427-435.1999 PMC Full Text View journal details
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