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PMID: 10318708 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Arabidopsis 22-kilodalton peroxisomal membrane protein. Nucleotide sequence analysis and biochemical characterization.

Plant physiology ·Vol. 120 ·No. 1 ·1999-05-00 ·Pages 309-20

Tugal HB, Pool M, Baker A

Abstract

We sequenced and characterized PMP22 (22-kD peroxisomal membrane protein) from Arabidopsis, which shares 28% to 30% amino acid identity and 55% to 57% similarity to two related mammalian peroxisomal membrane proteins, PMP22 and Mpv17. Subcellular fractionation studies confirmed that the Arabidopsis PMP22 is a genuine peroxisomal membrane protein. Biochemical analyses established that the Arabidopsis PMP22 is an integral membrane protein that is completely embedded in the lipid bilayer. In vitro import assays demonstrated that the protein is inserted into the membrane posttranslationally in the absence of ATP, but that ATP stimulates the assembly into the native state. Arabidopsis PMP22 is expressed in all organs of the mature plant and in tissue-cultured cells. Expression of PMP22 is not associated with a specific peroxisome type, as it is detected in seeds and throughout postgerminative growth as cotyledon peroxisomes undergo conversion from glyoxysomes to leaf-type peroxisomes. Although PMP22 shows increased accumulation during the growth of young seedlings, its expression is not stimulated by light.

MeSH Terms
Amino Acid Sequence Arabidopsis/chemistry,genetics,growth & development DNA, Complementary/genetics DNA, Plant/genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes, Plant Membrane Proteins/chemistry,genetics Microbodies/chemistry Molecular Sequence Data Plant Proteins/chemistry,genetics Sequence Homology, Amino Acid
Chemicals
DNA, Complementary DNA, Plant Membrane Proteins Plant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tugal H B
Centre for Plant Sciences, Leeds Institute for Plant Biotechnology and Agriculture, University of Leeds, Leeds LS2 9JT, United Kingdom.
Pool M
Baker A
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.120.1.309
Published
1999-05-00
Pages
309-20
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC59263
Subset
IM
Databases
GENBANK
AJ006053
External Links
PubMed source DOI: 10.1104/pp.120.1.309 PMC Full Text View journal details
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