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PMID: 10366590 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

p24 proteins and quality control of LIN-12 and GLP-1 trafficking in Caenorhabditis elegans.

The Journal of cell biology ·Vol. 145 ·No. 6 ·1999-06-14 ·Pages 1165-75

Wen C, Greenwald I

Abstract

Mutations in the Caenorhabditis elegans sel-9 gene elevate the activity of lin-12 and glp-1, which encode members of the LIN-12/NOTCH family of receptors. Sequence analysis indicates SEL-9 is one of several C. elegans p24 proteins. Allele-specific genetic interactions suggest that reducing sel-9 activity increases the activity of mutations altering the extracellular domains of LIN-12 or GLP-1. Reducing sel-9 activity restores the trafficking to the plasma membrane of a mutant GLP-1 protein that would otherwise accumulate within the cell. Our results suggest a role for SEL-9 and other p24 proteins in the negative regulation of transport of LIN-12 and GLP-1 to the cell surface, and favor a role for p24 proteins in a quality control mechanism for endoplasmic reticulum-Golgi transport.

MeSH Terms
Alleles Amino Acid Sequence Animals Biological Transport Caenorhabditis elegans/cytology,genetics,metabolism Caenorhabditis elegans Proteins Cell Lineage Cell Membrane/metabolism Cloning, Molecular Disorders of Sex Development Endoplasmic Reticulum/chemistry,metabolism Genes, Helminth/genetics,physiology Genes, Lethal/genetics Golgi Apparatus/chemistry,metabolism Helminth Proteins/chemistry,genetics,metabolism,physiology Membrane Glycoproteins/genetics,metabolism Membrane Proteins/genetics,metabolism Molecular Sequence Data Mutation Phenotype Receptors, Notch Sequence Homology, Amino Acid Stem Cells/cytology Suppression, Genetic/genetics
Chemicals
Caenorhabditis elegans Proteins Glp-1 protein, C elegans Helminth Proteins Lin-12 protein, C elegans Membrane Glycoproteins Membrane Proteins Receptors, Notch SEL-9 protein, C elegans
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wen C
Department of Biochemistry and Molecular Biophysics, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA.
Greenwald I
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.145.6.1165
Published
1999-06-14
Pages
1165-75
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2133156
Subset
IM
Grants
NIGMS NIH HHS · GM37602 · United States
External Links
PubMed source DOI: 10.1083/jcb.145.6.1165 PMC Full Text View journal details
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