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PMID: 1153334 Published · ppublish English Journal Article

Hybridization of polyuridylic acid to human DNA immobilized onto nitrocellulose filters.

Nucleic acids research ·Vol. 2 ·No. 7 ·1975-07-00 ·Pages 1163-76

Pero RW, Bryngelsson T, Bryngelsson C, Deutsch A, Nordén A, Norgren A

Abstract

The level of deoxyadenylate (da) regions in human DNA was estimated from formation of poly(U)-poly(da) triplexes on nitrocellulose filters that were RNAase resistant. The (dA) rich sequences were determined following mild ribonuclease treatment of the poly(U)-DNA hybrids (5 mug/ml at 25 degreesC for 30 min), where as exhaustive ribonuclease treatment (5 mug/ml at 25 degrees C for 6 hr) estimated the more (dA) pure sequences. The level of (dA) rich regions was 0.39% of the DNA and for the more (dA) pure regions it was 0.07%. The (dA) regions were widely distributed throughout human DNA regardless of base composition or sequence repetition. However, a concentration of (dA) regions into main band CsC1 gradient fractions of DNA and into repeated DNA was observed.

MeSH Terms
Adenine Nucleotides/analysis Breast/analysis Centrifugation, Density Gradient Chromatography DNA/analysis,blood,isolation & purification Female Humans Hydroxyapatites Lymphocytes/analysis Nucleic Acid Hybridization Pancreas/enzymology Placenta/analysis Poly U Pregnancy Ribonucleases Ultrafiltration
Chemicals
Adenine Nucleotides Hydroxyapatites Poly U DNA Ribonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Pero R W
Bryngelsson T
Bryngelsson C
Deutsch A
Nordén A
Norgren A
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/2.7.1163
Published
1975-07-00
Pages
1163-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343503
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/2.7.1163 PMC Full Text View journal details
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