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PMID: 118926 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Purification and characterization of a hemolysin produced by Vibrio cholerae biotype El Tor: another toxic substance produced by cholera vibrios.

Infection and immunity ·Vol. 26 ·No. 3 ·1979-12-00 ·Pages 1020-7

Honda T, Finkelstein RA

Abstract

A thermolabile direct hemolysin from an El Tor cholera vibrio strain has been isolated and partially characterized as a simple protein of ca. 20,000 molecular weight. In addition to its hemolytic activity, the hemolysin is cytotoxic, cardiotoxic, and rapidly lethal. In these respects it resembles the thermostable direct hemolysin/cytotoxin/cardiotoxin/lethal toxin of Vibrio parahaemolyticus and certain other bacterial hemolysins, although there are other significant differences. Because identical diseases are produced by both hemolytic and nonhemolytic cholera vibrios, the El Tor hemolysin may be presumed to be pathogenetically irrelevant. These observations raise the question of "When is a toxic substance also a toxin?"

MeSH Terms
Adrenal Glands/drug effects Animals Cells, Cultured Chromatography, Gel Culture Media Electrophoresis, Polyacrylamide Gel Heart/drug effects Hemolysin Proteins/analysis,immunology,isolation & purification Hot Temperature Immunodiffusion Immunoelectrophoresis Molecular Weight Organ Culture Techniques Rats Vibrio cholerae/metabolism
Chemicals
Culture Media Hemolysin Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Honda T
Finkelstein R A
References (37)
37 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.26.3.1020-1027.1979
Published
1979-12-00
Pages
1020-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414722
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.26.3.1020-1027.1979 PMC Full Text View journal details
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