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PMID: 1373379 Published · ppublish English Comparative Study Journal Article

Analysis of the BiP gene and identification of an ER retention signal in Schizosaccharomyces pombe.

The EMBO journal ·Vol. 11 ·No. 4 ·1992-04-00 ·Pages 1583-91

Pidoux AL, Armstrong J

Abstract

We have cloned the gene for the resident luminal ER protein BiP from the fission yeast, Schizosaccharomyces pombe. The predicted protein product is equally divergent from the budding yeast and mammalian homologues. Disruption of the BiP gene in S. pombe is lethal and BiP mRNA levels are regulated by a variety of stresses including heat shock. Immunofluorescence of cells expressing an epitope-tagged BiP protein show it to be localized to the nuclear envelope, around the cell periphery and in a reticular structure through the cytoplasm. Unexpectedly, we find the BiP protein contains an N-linked glycosylation site which can be utilized. The C-terminal four amino acids of BiP are Ala-Asp-Glu-Leu, a new variant of the XDEL sequence found at the C-termini of luminal endoplasmic reticulum proteins. To determine whether this sequence acts as a sorting signal in S.pombe we expressed an acid phosphatase fusion protein extended at its C-terminus with the amino acids ADEL. Analysis of the sorting of this fusion protein indicates that the ADEL sequence is sufficient to cause the retention of proteins in the endoplasmic reticulum. The sequences DDEL, HDEL and KDEL can also direct ER-retention of acid phosphatase in S.pombe.

Related Genes
BiP pho4
MeSH Terms
Acid Phosphatase/genetics,metabolism Amino Acid Sequence Animals Base Sequence Blotting, Western Carrier Proteins/analysis,genetics Cloning, Molecular DNA, Fungal/genetics,isolation & purification Endoplasmic Reticulum/metabolism Endoplasmic Reticulum Chaperone BiP Epitopes/analysis Fluorescent Antibody Technique Genes, Fungal Glycosylation Heat-Shock Proteins/genetics Molecular Chaperones Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction RNA, Messenger/genetics,metabolism Rats Restriction Mapping Schizosaccharomyces/cytology,genetics,metabolism Sequence Homology, Nucleic Acid
Chemicals
Carrier Proteins DNA, Fungal Endoplasmic Reticulum Chaperone BiP Epitopes Heat-Shock Proteins Molecular Chaperones Oligodeoxyribonucleotides RNA, Messenger Acid Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pidoux A L
Membrane Molecular Biology Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London, UK.
Armstrong J
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-04-00
Pages
1583-91
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556607
Subset
IM
Databases
GENBANK
X64416, X67718, X67719, X67720, X67721, X67722, X67723, X67724, X67725, X67726
External Links
PubMed source PMC Full Text View journal details
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