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PMID: 1388268 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Independent deposition of heterogeneous nuclear ribonucleoproteins and small nuclear ribonucleoprotein particles at sites of transcription.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 18 ·1992-09-15 ·Pages 8409-13

Amero SA, Raychaudhuri G, Cass CL, van Venrooij WJ, Habets WJ, Krainer AR, Beyer AL

Abstract

The major nuclear ribonucleoproteins (RNPs) involved in pre-mRNA processing are classified in broad terms either as small nuclear RNPs (snRNPs), which are major participants in the splicing reaction, or heterogeneous nuclear RNPs (hnRNPs), which traditionally have been thought to function in general pre-mRNA packaging. We obtained antibodies that recognize these two classes of RNP in Drosophila melanogaster. Using a sequential immunostaining technique to compare directly the distribution of these RNPs on Drosophila polytene chromosomes, we found that the two patterns were very similar qualitatively but not quantitatively, arguing for the independent deposition of the two RNP types and supporting a role for hnRNP proteins, but not snRNPs, in general transcript packaging.

MeSH Terms
Animals Drosophila melanogaster Fluorescent Antibody Technique Heterogeneous-Nuclear Ribonucleoproteins Nucleic Acid Precursors/metabolism RNA Processing, Post-Transcriptional RNA, Heterogeneous Nuclear/metabolism RNA, Messenger/metabolism Ribonucleoproteins/metabolism Ribonucleoproteins, Small Nuclear Transcription, Genetic
Chemicals
Heterogeneous-Nuclear Ribonucleoproteins Nucleic Acid Precursors RNA, Heterogeneous Nuclear RNA, Messenger Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Amero S A
Department of Microbiology, University of Virginia School of Medicine, Charlottesville 22908.
Raychaudhuri G
Cass C L
van Venrooij W J
Habets W J
Krainer A R
Beyer A L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.18.8409
Published
1992-09-15
Pages
8409-13
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49929
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.18.8409 PMC Full Text View journal details
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