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PMID: 2104660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Drosophila Hrb98DE locus encodes four protein isoforms homologous to the A1 protein of mammalian heterogeneous nuclear ribonucleoprotein complexes.

Molecular and cellular biology ·Vol. 10 ·No. 1 ·1990-01-00 ·Pages 316-23

Haynes SR, Raychaudhuri G, Beyer AL

Abstract

The Drosophila Hrb98DE locus encodes proteins that are highly homologous to the mammalian A1 protein, a major component of heterogeneous nuclear ribonucleoprotein (RNP) particles. The Hrb98DE locus is transcribed throughout development, with the highest transcript levels found in ovaries, early embryos, and pupae. Eight different transcripts are produced by the use of combinations of alternative promoters, exons, and splice acceptor sites; the various species are not all equally abundant. The 3'-most exon is unusual in that it is completely noncoding. These transcripts can potentially generate four protein isoforms that differ in their N-terminal 16 to 21 amino acids but are identical in the remainder of the protein, including the RNP consensus motif domain and the glycine-rich domain characteristic of the mammalian A1 protein. We suggest that these sequence differences could affect the affinities of the proteins for RNA or other protein components of heterogeneous nuclear RNP complexes, leading to differences in function.

MeSH Terms
Age Factors Amino Acid Sequence Animals Base Sequence Blotting, Northern Chromosome Mapping Drosophila melanogaster/genetics,growth & development Gene Expression Regulation Genes Molecular Sequence Data RNA Splicing RNA, Messenger/genetics Restriction Mapping Ribonucleoproteins/genetics
Chemicals
RNA, Messenger Ribonucleoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Haynes S R
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Raychaudhuri G
Beyer A L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.1.316-323.1990
Published
1990-01-00
Pages
316-23
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360745
Subset
IM
Grants
NIGMS NIH HHS · GM39271 · United States
Databases
GENBANK
M15766, M28870, M28871, M28872
External Links
PubMed source DOI: 10.1128/mcb.10.1.316-323.1990 PMC Full Text View journal details
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