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PMID: 1404596 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mammalian subtilisin-related proteinases in cleavage activation of the paramyxovirus fusion glycoprotein: superiority of furin/PACE to PC2 or PC1/PC3.

Journal of virology ·Vol. 66 ·No. 11 ·1992-11-00 ·Pages 6391-7

Gotoh B, Ohnishi Y, Inocencio NM, Esaki E, Nakayama K, Barr PJ, Thomas G, Nagai Y

Abstract

The fusion glycoprotein precursor of Newcastle disease virus is ubiquitously cleaved in the constitutive secretory pathway if it possesses an oligobasic cleavage motif (RRQR/KR), whereas the precursor is refractory to cleavage if the motif is monobasic (GR/KQGR). We examined the cleavage activity of the mammalian subtilisin-related proteinases furin/PACE, PC2, and PC1/PC3, which are thought to be responsible for proprotein processing in either the constitutive (furin/PACE) or the regulated (PC2 and PC1/PC3) secretory pathway, for the viral precursors with different cleavage motifs. Only furin/PACE was fully capable of cleaving the precursors with the oligobasic motif. PC2 and PC1/PC3 were incapable or only partially capable of cleaving at this motif. None of the proteinases cleaved the monobasic motif. These results suggest involvement of furin/PACE in viral protein processing in the constitutive secretory pathway.

MeSH Terms
Amino Acid Sequence Animals Furin Molecular Sequence Data Newcastle disease virus/metabolism Proprotein Convertase 2 Protein Precursors/metabolism Protein Processing, Post-Translational Serine Endopeptidases/pharmacology Substrate Specificity Subtilisins/pharmacology Viral Fusion Proteins/drug effects
Chemicals
Protein Precursors Viral Fusion Proteins Serine Endopeptidases Subtilisins Furin Proprotein Convertase 2
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gotoh B
Radioisotope Center Medical Division, Nagoya University School of Medicine, Japan.
Ohnishi Y
Inocencio N M
Esaki E
Nakayama K
Barr P J
Thomas G
Nagai Y
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.11.6391-6397.1992
Published
1992-11-00
Pages
6391-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240131
Subset
IM
Grants
NIDDK NIH HHS · DK-37274 · United States
NIDDK NIH HHS · DK-44629 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.11.6391-6397.1992 PMC Full Text View journal details
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