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PMID: 1409590 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Emr, an Escherichia coli locus for multidrug resistance.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 19 ·1992-10-01 ·Pages 8938-42

Lomovskaya O, Lewis K

Abstract

An Escherichia coli chromosomal DNA fragment cloned on a multicopy plasmid conferred resistance to carbonylcyanide m-chlorophenylhydrazone, nalidixic acid, and a number of other toxic compounds. The sequence of the cloned emr locus located at minute 57.5 of the chromosome revealed two open reading frames, emrA and emrB. emrB encodes a highly hydrophobic 56.2-kDa peptide, with 14 potential alpha-helices to span the inner membrane. The peptide is homologous to QacA, a multidrug-resistant pump from Staphylococcus aureus, and belongs to a gene family that includes tetracycline-resistant pumps of Gram-positive bacteria and the galactose/H+ symporter of E. coli. emrA encodes a putative 42.7-kDa peptide containing a single hydrophobic domain and a large C-terminal hydrophilic domain. An active pho-fusion to the C domain suggested that EmrA is a membrane protein. Disruption of emrB significantly increased sensitivity of cells to uncouplers. The cellular content of uncoupler increased in the order: overexpressed emrB cells greater than wild type greater than emrB-.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Carbonyl Cyanide m-Chlorophenyl Hydrazone/pharmacology Carrier Proteins/genetics Chromosomes, Bacterial/physiology Drug Resistance, Microbial/genetics Escherichia coli/drug effects,genetics,growth & development Escherichia coli Proteins Genes, Bacterial Kinetics Membrane Proteins/genetics Membrane Transport Proteins Molecular Sequence Data Multigene Family Nalidixic Acid/pharmacology Nitriles/pharmacology Open Reading Frames Polymerase Chain Reaction Protein Biosynthesis RNA, Messenger/genetics Restriction Mapping Sequence Homology, Amino Acid Staphylococcus aureus/genetics Tetracycline Resistance/genetics Transcription, Genetic
Chemicals
Bacterial Proteins Carrier Proteins EmrA protein, E coli Escherichia coli Proteins Membrane Proteins Membrane Transport Proteins Nitriles RNA, Messenger mesoxalonitrile qacA protein, Staphylococcus aureus emrA protein, Bacteria VceB protein, Vibrio cholerae Nalidixic Acid Carbonyl Cyanide m-Chlorophenyl Hydrazone
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lomovskaya O
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Lewis K
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.19.8938
Published
1992-10-01
Pages
8938-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50039
Subset
IM
Databases
GENBANK
M86657
External Links
PubMed source DOI: 10.1073/pnas.89.19.8938 PMC Full Text View journal details
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