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PMID: 1429443 Published · ppublish English Journal Article

Regulation of the BamHI restriction-modification system by a small intergenic open reading frame, bamHIC, in both Escherichia coli and Bacillus subtilis.

Journal of bacteriology ·Vol. 174 ·No. 22 ·1992-11-00 ·Pages 7194-201

Ives CL, Nathan PD, Brooks JE

Abstract

BamHI, from Bacillus amyloliquefaciens H, is a type II restriction-modification system recognizing and cleaving the sequence G--GATCC. The BamHI restriction-modification system contains divergently transcribed endonuclease and methylase genes along with a small open reading frame oriented in the direction of the endonuclease gene. The small open reading frame has been designated bamHIC (for BamHI controlling element). It acts as both a positive activator of endonuclease expression and a negative repressor of methylase expression of BamHI clones in Escherichia coli. Methylase activity increased 15-fold and endonuclease activity decreased 100-fold when bamHIC was inactivated. The normal levels of activity for both methylase and endonuclease were restored by supplying bamHIC in trans. The BamHI restriction-modification system was transferred into Bacillus subtilis, where bamHIC also regulated endonuclease expression when present on multicopy plasmid vectors or integrated into the chromosome. In B. subtilis, disruption of bamHIC caused at least a 1,000-fold decrease in endonuclease activity; activity was partially restored by supplying bamHIC in trans.

Related Genes
BaamHI bamHIC
MeSH Terms
Bacillus subtilis/enzymology,genetics Cloning, Molecular DNA, Bacterial/genetics,isolation & purification Deoxyribonuclease BamHI/genetics,isolation & purification,metabolism Escherichia coli/enzymology,genetics Gene Expression Gene Expression Regulation, Bacterial Gene Expression Regulation, Enzymologic Genes, Bacterial Introns Kinetics Open Reading Frames Plasmids Restriction Mapping
Chemicals
DNA, Bacterial Deoxyribonuclease BamHI
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ives C L
New England Biolabs, Beverly, Massachusetts 01915.
Nathan P D
Brooks J E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.22.7194-7201.1992
Published
1992-11-00
Pages
7194-201
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207411
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.22.7194-7201.1992 PMC Full Text View journal details
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