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PMID: 15166316 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Dissection of the mammalian midbody proteome reveals conserved cytokinesis mechanisms.

Science (New York, N.Y.) ·Vol. 305 ·No. 5680 ·2004-07-02 ·Pages 61-6

Skop AR, Liu H, Yates J, Meyer BJ, Heald R

Abstract

Cytokinesis is the essential process that partitions cellular contents into daughter cells. To identify and characterize cytokinesis proteins rapidly, we used a functional proteomic and comparative genomic strategy. Midbodies were isolated from mammalian cells, proteins were identified by multidimensional protein identification technology (MudPIT), and protein function was assessed in Caenorhabditis elegans. Of 172 homologs disrupted by RNA interference, 58% displayed defects in cleavage furrow formation or completion, or germline cytokinesis. Functional dissection of the midbody demonstrated the importance of lipid rafts and vesicle trafficking pathways in cytokinesis, and the utilization of common membrane cytoskeletal components in diverse morphogenetic events in the cleavage furrow, the germline, and neurons.

MeSH Terms
Animals CHO Cells Caenorhabditis elegans/cytology,genetics,physiology Carrier Proteins/analysis,isolation & purification,physiology Cell Cycle/physiology Cell Division Cell Fractionation Cell Membrane/physiology Computational Biology Cricetinae Cytoskeletal Proteins/analysis,isolation & purification,physiology Cytoskeleton/physiology Germ Cells/physiology HeLa Cells Humans Membrane Microdomains/physiology Morphogenesis Organelles/chemistry,physiology Protein Transport Proteins/analysis,isolation & purification,physiology Proteome/analysis Proteomics Signal Transduction Spindle Apparatus/physiology,ultrastructure
Chemicals
Carrier Proteins Cytoskeletal Proteins Proteins Proteome
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Skop Ahna R
Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA. [email protected]
Liu Hongbin
Yates John
Meyer Barbara J
Heald Rebecca
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Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
1095-9203
DOI
10.1126/science.1097931
Email
[email protected]
Published
2004-07-02
Epub
2004-00-27
Pages
61-6
Language
English
Region
United States
NLM ID
0404511
PMCID
PMC3679889
Subset
IM
Grants
NIGMS NIH HHS · F32 GM064159-02 · United States
NIGMS NIH HHS · F32 GM064159-03 · United States
NCRR NIH HHS · P41 RR011823 · United States
NIGMS NIH HHS · F32 GM064159-01 · United States
NIGMS NIH HHS · F32 GM64159-01 · United States
NCRR NIH HHS · RR1823 · United States
NIGMS NIH HHS · F32 GM064159 · United States
External Links
PubMed source DOI: 10.1126/science.1097931 PMC Full Text View journal details
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