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PMID: 1569026 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Corynebacterium glutamicum aecD gene encodes a C-S lyase with alpha, beta-elimination activity that degrades aminoethylcysteine.

Journal of bacteriology ·Vol. 174 ·No. 9 ·1992-05-00 ·Pages 2968-77

Rossol I, Pühler A

Abstract

S-(beta-Aminoethyl)-cysteine (AEC) resistance was achieved in Corynebacterium glutamicum by cloning a chromosomal 1.5-kb EcoRV-BglII DNA fragment on a multicopy plasmid. DNA sequence analysis of the 1.5-kb DNA fragment revealed an open reading frame (ORF326) which represents the AEC resistance gene, designated aecD. The aecD gene directs the synthesis of a 36-kDa protein which was visualized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The aecD gene is a nonessential gene and mediates AEC resistance only in an amplified state. C. glutamicum strains harboring an amplified aecD gene can utilize AEC as an alternative nitrogen source, indicating that the AEC resistance mechanism is due to AEC degradation. Since the AEC degradation products analyzed by high-pressure liquid chromatography were found to be pyruvate and aminoethanethiol (cysteamine), it was concluded that the aecD gene encodes a C-S lyase with alpha, beta-elimination activity. Besides AEC, the C-S lyase was also able to use cysteine, cystine, and cystathionine as substrates.

Related Genes
aecD
MeSH Terms
Amino Acid Sequence Base Sequence Corynebacterium/enzymology,genetics Cysteamine/metabolism Cysteine/analogs & derivatives,metabolism Drug Resistance, Microbial/genetics Gene Amplification Lyases/genetics Molecular Sequence Data Pyruvates/metabolism Substrate Specificity
Chemicals
Pyruvates S-2-aminoethyl cysteine Cysteamine Lyases carbon-sulfur lyase Cysteine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rossol I
Department of Genetics, University of Bielefeld, Germany.
Pühler A
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.9.2968-2977.1992
Published
1992-05-00
Pages
2968-77
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205951
Subset
IM
Databases
GENBANK
M79367, M79368, M79369, M79370, M79371, M79372, M87491, M87492, M89931, S97385
External Links
PubMed source DOI: 10.1128/jb.174.9.2968-2977.1992 PMC Full Text View journal details
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