Home LiteratureArticle Details
PMID: 1569959 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Expression cloning of a novel zinc finger protein that binds to the c-fos serum response element.

Molecular and cellular biology ·Vol. 12 ·No. 5 ·1992-05-00 ·Pages 2432-43

Attar RM, Gilman MZ

Abstract

Induction of c-fos transcription by serum growth factors requires the serum response element (SRE). The SRE is a multifunctional element which responds to several positively and negatively acting signals. To identify cellular proteins that might mediate functions of the SRE, we screened a human cDNA expression library with an SRE probe. We report the isolation and characterization of SRE-ZBP, a previously unidentified SRE-binding protein. SRE-ZBP is a member of the C2H2 zinc finger family of proteins exemplified by TFIIIA and the Drosophila Krüppel protein. The seven tandemly repeated zinc finger motifs in SRE-ZBP are sufficient for high-affinity binding to the SRE. We show that SRE-ZBP is a nuclear protein and identify a candidate cellular protein encoded by the SRE-ZBP gene. Because we cannot detect any DNA-binding activity attributable to the endogenous protein, we propose that SRE-ZBP activity may be subject to posttranslational regulation. Like c-fos mRNA, SRE-ZBP mRNA is serum inducible in HeLa cells, but with slower kinetics. The role of SRE-ZBP in the regulation of c-fos transcription remains unestablished, but this protein binds to a region of the SRE where mutations lead to derepression.

Related Genes
c-fos
MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular/methods DNA/genetics,isolation & purification DNA Probes DNA-Binding Proteins/metabolism Escherichia coli/genetics Fluorescent Antibody Technique Gene Library Genes, fos Humans Immune Sera Molecular Sequence Data Nuclear Proteins/metabolism Oligodeoxyribonucleotides Plasmids Recombinant Fusion Proteins/metabolism Sequence Homology, Nucleic Acid Serum Response Factor Substrate Specificity Transcription Factors/metabolism Transfection Zinc Fingers/genetics,physiology beta-Galactosidase/genetics,metabolism
Chemicals
DNA Probes DNA-Binding Proteins Immune Sera Nuclear Proteins Oligodeoxyribonucleotides Recombinant Fusion Proteins Serum Response Factor Transcription Factors DNA beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Attar R M
Cold Spring Harbor Laboratory, New York 11724.
Gilman M Z
References (75)
75 references, click to expand
  1. Occupation of the c-fos serum response element in vivo by a multi-protein complex is unaltered by growth factor induction.
    Nature. 1989 Jul 6;340(6228):68-70 PMID: 2786995
  2. Identification of c-fos sequences involved in induction by insulin and phorbol esters.
    J Biol Chem. 1988 Feb 5;263(4):1611-4 PMID: 3276673
  3. c-fos sequence necessary for basal expression and induction by epidermal growth factor, 12-O-tetradecanoyl phorbol-13-acetate and the calcium ionophore.
    Mol Cell Biol. 1987 Oct;7(10):3490-502 PMID: 3119989
  4. Inducible binding of a factor to the c-fos enhancer.
    Cell. 1986 Dec 5;47(5):777-84 PMID: 3096578
  5. Repression of c-fos transcription is mediated through p67SRF bound to the SRE.
    EMBO J. 1989 Sep;8(9):2567-74 PMID: 2511007
  6. Autoregulation of fos: the dyad symmetry element as the major target of repression.
    EMBO J. 1989 Sep;8(9):2559-66 PMID: 2511006
  7. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
    Gene. 1988 Jul 15;67(1):31-40 PMID: 3047011
  8. Activation of the c-fos gene by UV and phorbol ester: different signal transduction pathways converge to the same enhancer element.
    Oncogene. 1988 Sep;3(3):301-11 PMID: 2849742
  9. Point mutational analysis of the human c-fos serum response factor binding site.
    Nucleic Acids Res. 1989 Feb 11;17(3):1177-95 PMID: 2493627
  10. Two mouse genes encoding potential transcription factors with identical DNA-binding domains are activated by growth factors in cultured cells.
    Proc Natl Acad Sci U S A. 1988 Jul;85(13):4691-5 PMID: 3133658
  11. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method.
    Mol Cell Biol. 1988 May;8(5):2159-65 PMID: 2455217
  12. Specific and ubiquitous expression of different Zn finger protein genes in the mouse.
    Nucleic Acids Res. 1988 Nov 11;16(21):9995-10011 PMID: 3143103
  13. A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):7857-61 PMID: 3141919
  14. Fos C-terminal mutations block down-regulation of c-fos transcription following serum stimulation.
    EMBO J. 1988 Dec 20;7(13):4193-202 PMID: 3149583
  15. A conserved family of nuclear proteins containing structural elements of the finger protein encoded by Krüppel, a Drosophila segmentation gene.
    Cell. 1986 Dec 26;47(6):1025-32 PMID: 3096579
  16. In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage.
    Genes Dev. 1988 Jul;2(7):801-6 PMID: 3061875
  17. The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP.
    Genes Dev. 1988 Apr;2(4):394-402 PMID: 2453393
  18. Multiple sequence elements of a single functional class are required for cyclic AMP responsiveness of the mouse c-fos promoter.
    Mol Cell Biol. 1989 Oct;9(10):4272-81 PMID: 2555687
  19. DNA binding site of the growth factor-inducible protein Zif268.
    Proc Natl Acad Sci U S A. 1989 Nov;86(22):8737-41 PMID: 2510170
  20. c-fos proto-oncogene expression in the nervous system during mouse development.
    Mol Cell Biol. 1989 May;9(5):2269-72 PMID: 2501666
  21. Cross-binding of factors to functionally different promoter elements in c-fos and skeletal actin genes.
    Mol Cell Biol. 1989 May;9(5):2191-201 PMID: 2501661
  22. cAMP stimulates the C/EBP-related transcription factor rNFIL-6 to trans-locate to the nucleus and induce c-fos transcription.
    Genes Dev. 1991 Oct;5(10):1754-66 PMID: 1655570
  23. Binding of the Wilms' tumor locus zinc finger protein to the EGR-1 consensus sequence.
    Science. 1990 Nov 30;250(4985):1259-62 PMID: 2244209
  24. Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein.
    Cell. 1991 Oct 18;67(2):377-88 PMID: 1655281
  25. Members of the zinc finger protein gene family sharing a conserved N-terminal module.
    Nucleic Acids Res. 1991 Oct 25;19(20):5661-7 PMID: 1945843
  26. Segments of the POU domain influence one another's DNA-binding specificity.
    Mol Cell Biol. 1992 Feb;12(2):455-67 PMID: 1732727
  27. Isolation, characterization, and expression of the murine Wilms' tumor gene (WT1) during kidney development.
    Mol Cell Biol. 1991 Mar;11(3):1707-12 PMID: 1671709
  28. Activation and repression of transcription by the gap proteins hunchback and Krüppel in cultured Drosophila cells.
    Genes Dev. 1991 Feb;5(2):254-64 PMID: 1671661
  29. Cell-type specific multiprotein complex formation over the c-fos serum response element in vivo: ternary complex formation is not required for the induction of c-fos.
    Nucleic Acids Res. 1991 Jul 11;19(13):3607-11 PMID: 1906604
  30. Distinct protein targets for signals acting at the c-fos serum response element.
    Science. 1991 Jan 11;251(4990):189-92 PMID: 1898992
  31. Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A.
    Science. 1991 May 10;252(5007):809-17 PMID: 2028256
  32. Glass encodes a site-specific DNA-binding protein that is regulated in response to positional signals in the developing Drosophila eye.
    Genes Dev. 1991 Apr;5(4):583-93 PMID: 2010085
  33. The SRE: a growth factor responsive transcriptional regulator.
    Semin Cancer Biol. 1990 Feb;1(1):47-58 PMID: 2133110
  34. The primary structure of the murine multifinger gene mKr2 and its specific expression in developing and adult neurons.
    EMBO J. 1988 May;7(5):1345-53 PMID: 3409867
  35. Complexity of the early genetic response to growth factors in mouse fibroblasts.
    Mol Cell Biol. 1988 May;8(5):2140-8 PMID: 2898731
  36. Potential metal-binding domains in nucleic acid binding proteins.
    Science. 1986 Apr 25;232(4749):485-7 PMID: 2421409
  37. Microinjection of the catalytic subunit of cAMP-dependent protein kinase induces expression of the c-fos gene.
    Cold Spring Harb Symp Quant Biol. 1988;53 Pt 1:85-90 PMID: 2855494
  38. Transcriptional autoregulation of the proto-oncogene fos.
    Nature. 1988 Jul 28;334(6180):314-9 PMID: 2839774
  39. Multiple protein-binding sites in the 5'-flanking region regulate c-fos expression.
    Mol Cell Biol. 1986 Dec;6(12):4305-16 PMID: 3025651
  40. Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA.
    Cell. 1988 Feb 12;52(3):415-23 PMID: 2964277
  41. Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of the transferrin receptor gene.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):6741-4 PMID: 2995982
  42. Intracellular mediators of c-fos induction.
    Cold Spring Harb Symp Quant Biol. 1988;53 Pt 2:761-7 PMID: 3151188
  43. A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells.
    EMBO J. 1988 Jan;7(1):29-35 PMID: 3129290
  44. A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization.
    Cell. 1988 Apr 8;53(1):37-43 PMID: 3127059
  45. Calcium and growth factor pathways of c-fos transcriptional activation require distinct upstream regulatory sequences.
    Mol Cell Biol. 1988 Jul;8(7):2787-96 PMID: 3136322
  46. Transcription activation by serum, PDGF, and TPA through the c-fos DSE: cell type specific requirements for induction.
    Oncogene. 1989 Jan;4(1):3-11 PMID: 2915902
  47. Two distinct cellular phosphoproteins bind to the c-fos serum response element.
    EMBO J. 1989 Jun;8(6):1785-92 PMID: 2504586
  48. The ability of a ternary complex to form over the serum response element correlates with serum inducibility of the human c-fos promoter.
    Cell. 1989 Feb 24;56(4):563-72 PMID: 2492906
  49. The products of the Drosophila gap genes hunchback and Krüppel bind to the hunchback promoters.
    Nature. 1989 Sep 28;341(6240):335-7 PMID: 2797150
  50. Negative regulation of serum-responsive enhancer elements.
    Nature. 1989 Jul 6;340(6228):64-6 PMID: 2739725
  51. Segment-specific expression of a zinc-finger gene in the developing nervous system of the mouse.
    Nature. 1989 Feb 2;337(6206):461-4 PMID: 2915691
  52. The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
    Genes Dev. 1988 Dec;2(12A):1582-99 PMID: 2905684
  53. Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element.
    Cell. 1988 Dec 23;55(6):989-1003 PMID: 3203386
  54. Cloning of a lymphoid-specific cDNA encoding a protein binding the regulatory octamer DNA motif.
    Science. 1988 Jul 29;241(4865):577-80 PMID: 3399892
  55. Analysis of Fos protein complexes and Fos-related antigens by high-resolution two-dimensional gel electrophoresis.
    Oncogene. 1987 May;1(2):213-21 PMID: 3325882
  56. Isolation of cDNA encoding transcription factor Sp1 and functional analysis of the DNA binding domain.
    Cell. 1987 Dec 24;51(6):1079-90 PMID: 3319186
  57. Identification of a protein-binding site that mediates transcriptional response of the c-fos gene to serum factors.
    Cell. 1986 Aug 15;46(4):567-74 PMID: 3524858
  58. Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1234-8 PMID: 3469665
  59. Xenopus cytoskeletal actin and human c-fos gene promoters share a conserved protein-binding site.
    EMBO J. 1987 Mar;6(3):667-73 PMID: 3582369
  60. Mutation of the c-fos gene dyad symmetry element inhibits serum inducibility of transcription in vivo and the nuclear regulatory factor binding in vitro.
    Mol Cell Biol. 1987 Mar;7(3):1217-25 PMID: 3561415
  61. A multigene family encoding several "finger" structures is present and differentially active in mammalian genomes.
    Cell. 1987 Mar 13;48(5):771-8 PMID: 3815523
  62. Molecular genetics of Krüppel, a gene required for segmentation of the Drosophila embryo.
    Nature. 1985 Jan 3-9;313(5997):27-32 PMID: 3917552
  63. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  64. Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
    EMBO J. 1985 Jun;4(6):1609-14 PMID: 4040853
  65. Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation.
    Cell. 1990 Feb 9;60(3):375-86 PMID: 2302733
  66. Use of T7 RNA polymerase to direct expression of cloned genes.
    Methods Enzymol. 1990;185:60-89 PMID: 2199796
  67. Human proviral mRNAs down regulated in choriocarcinoma encode a zinc finger protein related to Krüppel.
    Mol Cell Biol. 1990 Aug;10(8):4401-5 PMID: 2115127
  68. Isolation and characterization of a zinc finger polypeptide gene at the human chromosome 11 Wilms' tumor locus.
    Cell. 1990 Feb 9;60(3):509-20 PMID: 2154335
  69. Drosophila Krüppel protein is a transcriptional repressor.
    Nature. 1990 Jul 5;346(6279):76-9 PMID: 2114551
  70. Demonstration in living cells of an intragenic negative regulatory element within the rodent c-fos gene.
    Cell. 1990 May 4;61(3):485-96 PMID: 2110508
  71. The inner core of the serum response element mediates both the rapid induction and subsequent repression of c-fos transcription following serum stimulation.
    Genes Dev. 1990 Feb;4(2):255-68 PMID: 2110922
  72. The GLI gene encodes a nuclear protein which binds specific sequences in the human genome.
    Mol Cell Biol. 1990 Feb;10(2):634-42 PMID: 2105456
  73. A mouse zinc finger gene which is transiently expressed during spermatogenesis.
    EMBO J. 1990 Jan;9(1):197-205 PMID: 2104800
  74. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  75. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.12.5.2432-2443.1992
Published
1992-05-00
Pages
2432-43
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364416
Subset
IM
Grants
NCI NIH HHS · CA45642 · United States
NCI NIH HHS · CA46370 · United States
Databases
GENBANK
M88579, Z11773
External Links
PubMed source DOI: 10.1128/mcb.12.5.2432-2443.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2